|
| Status |
Public on Nov 15, 2024 |
| Title |
Center1-068 |
| Sample type |
genomic |
| |
|
| Source name |
Peripheral Whole Blood
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Female
age: 58
treatment: Immunotherapy only
progression event: 1
days to progression: 94
death event: 1
days to death: 94
tmb: 8.365
pdl1 cps: 50
arraytypes: IlluminaHumanMethylationEPICv2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted and processed using the Zymo Quick DNA Miniprep Plus kit and quantified using a Qubit dsDNA Broad Range Assay kit (Thermo)
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium HumanMethylationEPICv1/v2 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
adult head and neck cancer peripheral blood before ICI treatment
|
| Data processing |
R studio
minfi Noob Processed Average Beta
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Sep 19, 2024 |
| Last update date |
Nov 15, 2024 |
| Contact name |
Ze Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Department |
Medical Oncology & Population Sciences
|
| Lab |
Baca Lab
|
| Street address |
375 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL33022 |
| Series (1) |
| GSE277573 |
Methylation cytometric pretreatment blood immune profiles with tumor mutation burden as prognostic indicators for survival outcomes in head and neck cancer patients on anti-PD-1 therapy |
|
