|
| Status |
Public on Oct 05, 2012 |
| Title |
Control cell, Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell type: MEF derived stable cell line
genotype/variation: Tet-off control cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using PureLink RNA Micro scale kit (Invitrogen). Microarray was performed with 300ng RNA. RNA integrety was tested with Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocoll
|
| |
|
| Hybridization protocol |
Affymetrix protocoll
|
| Scan protocol |
GeneChip 3000 7G(Affymetrix) was used. Array images were analysed for defects with the Expression Console software (Affymetrix).
|
| Data processing |
Raw data were normalized by GC RMA algorithm.GeneSpring GX 10.0 software (Agilent Technologies, Böblingen, Germany) was used for filtering and statistical data analysis. An unpaired T test FDR corrected by Benjamini Hochberg was carried out between control and experimental samples to test for differential expression.
|
| |
|
| Submission date |
Dec 23, 2011 |
| Last update date |
Oct 05, 2012 |
| Contact name |
Andreas Janzer |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Bonn
|
| Department |
Pathology
|
| Lab |
Kirfel
|
| Street address |
Sigmund Freud St. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE34691 |
Expression data from murine fibroblasts overexpressing Fbxl10 |
|
