|
| Status |
Public on Feb 01, 2012 |
| Title |
wildtype strong 1 |
| Sample type |
RNA |
| |
|
| Source name |
whole skin
|
| Organism |
Mus musculus |
| Characteristics |
genotype: tg/wt
treatment: CaNrf2
gender: female
background strain: FVB/N/C57BL/6 mixed
|
| Growth protocol |
3 female K5cre-caNrf2 (tg/tg) and 3 female K5cre-wt (tg/wt) mice of one litter were sacrificed at P2.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
back skin was cut out and snap frozen in N2, RNA was extracted using GSCN and phenol/chloroform
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
|
| Scan protocol |
Standard Affymetrix procedures using a Hewlett-Packard scanner
|
| Data processing |
Raw gene expression data generated by the GeneChip Operating Software (Affymetrix) were normalized for all probe sets on the array using the Robust Multichip Average (RMA) method.
|
| |
|
| Submission date |
Jan 17, 2012 |
| Last update date |
Feb 01, 2012 |
| Contact name |
Matthias Schäfer |
| E-mail(s) |
[email protected]
|
| Phone |
+41-44-633 3406
|
| Fax |
+41-44-633 1174
|
| Organization name |
ETH Zürich
|
| Department |
Department of Cell Biology
|
| Lab |
Werner
|
| Street address |
Schafmattstrasse 18
|
| City |
Zürich |
| State/province |
ZH |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35160 |
Functional analysis of the cytoprotective transcription factor Nrf2 in skin morphogenesis and disease: Identification of Nrf2 target genes |
|
