|
| Status |
Public on Jul 02, 2013 |
| Title |
sample.6 12.50 mg/kg DB[a,h]A |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
dose: 12.50 mg/kg DB[a,h]A
tissue: part of liver
gender: male
age: 10 weeks
genotype/variation: MutaMouse (transgenic mouse strain 40.6)
date of exposure: January 24- February 22
time post exposure: 3 days
|
| Treatment protocol |
10-week old male Muta™Mouse (i.e., transgenic mouse strain 40.6) were dosed daily via oral gavage for 28-days with DB[a,h]A dissolved in olive oil (6.25, 12.50 and 25 mg/kg body weight/day). Each dose group contained 5 animals; 5 animals were also dosed with a vehicle (olive oil) control. Following a 3 day expression period after the last treatment, mice were euthanized with isofluorane followed by cervical dislocation. Liver was then excised, flash-frozen in liquid nitrogen, and stored at -80ºC until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing the small fraction was isolated from each sample using the miRVana miRNA Isolation Kit (Ambion), Streetsville, ON, Canada). Quality was confirmed using the Agilent 2100 Bioanalyzer and RNA Nano 6000 chips.
|
| Label |
Cyanine 3-pCp
|
| Label protocol |
Total RNA samples were labelled using the Agilent miRNA Complete Labelling and Hybridisation Kit (Agilent Tech, Mississauga, ON, Canada).
|
| |
|
| Hybridization protocol |
Hybridisations were performed in Agilent SureHyb chambers for 20 hours at 55C and washed according to manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned at a resolution of 5 mm using an Agilent G2505B scanner.
|
| Data processing |
The log2 of median signal intensites were cyclic lowess normalized and the technical replicates were averaged using the median.
|
| |
|
| Submission date |
Jan 18, 2012 |
| Last update date |
Jul 02, 2013 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
|
| Street address |
50 Columbine
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL14972 |
| Series (2) |
| GSE35195 |
Lack of hepatic response of microRNA in mice following DB[a,h]A exposure (Agilent miRNA) |
| GSE35509 |
Lack of hepatic response of microRNA in mice following DB[a,h]A exposure |
|
