|
| Status |
Public on Nov 22, 2024 |
| Title |
Glioblastoma U251 methionine deprived culture 48h, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Glioblastoma U251 methionine deprived culture 48h
|
| Organism |
Homo sapiens |
| Characteristics |
human tumor cell line: Glioblastoma cell line U251
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Purify the labeled/amplified RNA and labeled cRNA were prepared according to the RNeasy Mini Kit (Qiagen p/n 74104) standard protocol from total RNA.
|
| |
|
| Hybridization protocol |
1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
Microarray were scanned using the Agilent Microarray Scanner (Agilent p/n G2565BA).
|
| Description |
Gene expression data from glioblastoma cell line of cellularisation.
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction (FE) software (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
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| |
|
| Submission date |
Nov 18, 2024 |
| Last update date |
Nov 22, 2024 |
| Contact name |
Hong Hu |
| E-mail(s) |
[email protected]
|
| Phone |
15685782660
|
| Organization name |
Harbin medical university
|
| Street address |
youzheng street
|
| City |
Harbin |
| State/province |
黑龙江 |
| ZIP/Postal code |
150001 |
| Country |
China |
| |
|
| Platform ID |
GPL26963 |
| Series (1) |
| GSE282194 |
Expression data from Methionine normal or deprived culture glioblastoma cell line U251 |
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