|
| Status |
Public on Aug 20, 2012 |
| Title |
brains and imaginal discs from Sak overexpressing 3rd instar Drosophila larvae, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
brains and imaginal discs from Drosophila 3rd instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Sak overexpression
tissue: brains and imaginal discs
developmental stage: 3rd instar larvae
|
| Treatment protocol |
Brains and imaginal discs were dissected in PBS under a dissecting scope and tissues from 10 larvae per sample were collected in a tube on dry ice.
|
| Growth protocol |
Flies were kept on standard fly food at 25 degrees. Third instar wandering larvae were used for dissections.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA using the Affymetrix 3’ IVT Express kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridised on Affymetrix Drosophila 2.0 GeneChips for 16 hrs at 45°C. Gene chips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip 3000 scanner.
|
| Description |
Gene expression data from mitotic Drosophila cells that have too many centrosomes
|
| Data processing |
The data was analysed using the Bioconductor package limma. Probe intensities from Affymetrix image files (.CEL files) were normalized using quantile normalisation, and expression signals of all probesets were calculated using GCRMA.
|
| |
|
| Submission date |
Jan 20, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Janina Baumbach |
| E-mail(s) |
[email protected]
|
| Phone |
441865275531
|
| Organization name |
University of Oxford
|
| Department |
Sir William Dunn School of Pathology
|
| Lab |
Raff lab
|
| Street address |
South Parks Road
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE35240 |
Gene expression in mitotic tissues of Drosophila larvae without centrosomes or too many centrosomes |
|
| Relations |
| Reanalyzed by |
GSE119084 |
