|
| Status |
Public on Nov 27, 2024 |
| Title |
E0del/+_SkM_2 |
| Sample type |
SRA |
| |
|
| Source name |
SkM
|
| Organism |
Mus musculus |
| Characteristics |
tissue: SkM
genotype: E0del/+
batch: 2
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted from mouse tissue using simple TRIzol method.
Libraries were constructed using Illumina's Nextera XT DNA Library Preparation Kit. Before libraries were constructed two rounds of poly-A selection were performed on total RNA before double stranded cDNA was synthesized. This cDNA was used as the templated for the library construction.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Library name: 1662_SkM
|
| Data processing |
Fastq files were aligned using STAR and counts files were generated. Downstream analysis was done using DESeq2.
Assembly: mm10
Supplementary files format and content: Processed data includes feature counts and bam file, which are both generated from STAR aligner.
|
| |
|
| Submission date |
Nov 22, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Seong Won Kim |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Street address |
77 Ave Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE282670 |
Regulation of sarcomere formation and function in the healthy heart requires a titin intronic enhancer |
|
| Relations |
| BioSample |
SAMN45005485 |
| SRA |
SRX26834219 |
