|
| Status |
Public on Jun 04, 2012 |
| Title |
ovary_15.5 dpc_1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse fetal ovary
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: C57BL/6
tissue: ovary
age: 15.5 days postcoitum
|
| Treatment protocol |
Gonads were dissected from 15.5 dpc mice.
|
| Growth protocol |
The C57BL/6 mice were obtained from a commercial breeder.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy kit (Qiagen) according to the
manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using MessageAmp aRNA Kit (Ambion) according to the manufacturer's instructions from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in a fluidics system.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from 15.5 dpc ovaries.
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS, version 1) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Feb 13, 2012 |
| Last update date |
Jun 04, 2012 |
| Contact name |
Makiko Tsutsumi |
| Organization name |
Fujita Health University
|
| Department |
ICMS
|
| Lab |
Div. Mol. Genet.
|
| Street address |
1-98 Dengakugakubo, Kutsukake-cho
|
| City |
Toyoake |
| State/province |
Aichi |
| ZIP/Postal code |
470-1192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35734 |
Screening of genes involved in chromosome segregation in meiosis I |
|
