|
| Status |
Public on Feb 04, 2023 |
| Title |
xenoNSP#1 |
| Sample type |
RNA |
| |
|
| Source name |
xenoNSP
|
| Organism |
Homo sapiens |
| Characteristics |
stem-like: no
lmp2a: no
cell line: HK1
|
| Growth protocol |
HK1 were maintained in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS) while C666-1 in fibronectin coated plate in RPMI 1640 medium supplemented with 15% FCS. For sphere-forming assay, C666-1 cells were seeded at 5000 cells/ well in 6-well low attachment plate in serum-free DMEM/F12 (1:1) media supplemented with 5ug/ml Insulin-Transferrin-Selenium-A, 20ng/ml rEGF, 10ng/ml bFGF, 0.4% BSA, 1xB-27 supplement. All cell lines were maintained in 50U/ml penicillin and 50 µg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All RNA samples were extracted by Trizol.
|
| Label |
Cy3
|
| Label protocol |
200ng RNA from each sample was labeled according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
All RNA samples were hybridized according to manufacturer's protocol.
|
| Scan protocol |
Scanned using GenePix 4000B
|
| Description |
Gene expression of the counterpart for NPC stem-like cells.
US45103080_251485038675_S01_GE2_107_Sep09_1_1.txt: Cy3
|
| Data processing |
Images from Agilent microarray were processed in Feature Extraction software. Data files from the Agilent chips were imported into GeneSpring as single color analysis and then the signal values transformed to the log base 2. Normalization was carried out with 75th percentile.
|
| |
|
| Submission date |
Feb 28, 2012 |
| Last update date |
Feb 04, 2023 |
| Contact name |
Lu Ping Tan |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute for Medical Research
|
| Department |
Molecular Pathology Unit
|
| Street address |
Jalan Pahang
|
| City |
Kuala Lumpur |
| ZIP/Postal code |
50588 |
| Country |
Malaysia |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
|
