|
| Status |
Public on Mar 01, 2012 |
| Title |
Akt tg P14 CD8 T cell_LCMV-infected SLEC |
| Sample type |
RNA |
| |
|
| Source name |
Akt tg P14, CD8 T cells, LCMV-infected, SLEC
|
| Organism |
Mus musculus |
| Characteristics |
background strain: P14
genotype: Akt transgenic
gender: female
age: 6-8 weeks
cell type: short-lived effector cells (SLECs)
treatment: LCMV infection
|
| Treatment protocol |
For LCMV-infected mice, mice were infected with 2 x 10^5 pfu of lymphocytic choriomeningitis virus (LCMV) Armstrong strain.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA, followed by clean-up with the RNeasy kit (Qiagen), was performed according to the manufacturers' instructions.
|
| Label |
Biotin
|
| Label protocol |
Labeling procedures were carried out according to the standard protocol from the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay.
|
| |
|
| Hybridization protocol |
All samples were hybridized to Mouse Gene 1.0 ST arrays at 45C for 16hrs following all procedures outlined in the GeneChip WT Sense Target Labeling Assay manual for Mouse Gene 1.0 ST arrays.
|
| Scan protocol |
GeneChips were scanned using the GC3000 G7 Scanner.
|
| Description |
Akt SLEC
|
| Data processing |
Data were extracted and processed using Affymetrix Command Console.
|
| |
|
| Submission date |
Feb 29, 2012 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Eui Ho Kim |
| E-mail(s) |
[email protected]
|
| Phone |
608-263-4715
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pathobiological Sciences
|
| Lab |
Dr. M. Suresh
|
| Street address |
2015 Linden Dr.
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE36168 |
Expression data from LCMV-infected P14 and Akt transgenic P14 CD8 T cells |
|
