|
Status |
Public on Mar 07, 2012 |
Title |
Keratinocytes_TNF_subject3 |
Sample type |
RNA |
|
|
Source name |
primary keratinocytes
|
Organism |
Homo sapiens |
Characteristics |
tissue: primary keratinocytes treatment: TNF-treated
|
Treatment protocol |
Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
|
Growth protocol |
Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using QIAGEN RNEasy mini kits
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
|
Description |
primary human keratinocytes treated with TNF
|
Data processing |
Robust Multichip Average (RMA)
|
|
|
Submission date |
Mar 05, 2012 |
Last update date |
Mar 07, 2012 |
Contact name |
William R Swindell |
E-mail(s) |
[email protected]
|
Organization name |
Harvard University
|
Department |
Genetics
|
Street address |
77 Avenue Louis Pasteur
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE36287 |
Expression data from primary human keratinocytes exposed to cytokines in vitro (IL-4, IL-13, IL-17A, IFN-alpha, IFN-gamma, TNF) |
|