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Sample GSM886444 Query DataSets for GSM886444
Status Public on Mar 07, 2012
Title Keratinocytes_TNF_subject3
Sample type RNA
 
Source name primary keratinocytes
Organism Homo sapiens
Characteristics tissue: primary keratinocytes
treatment: TNF-treated
Treatment protocol Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
Growth protocol Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
Extracted molecule total RNA
Extraction protocol RNA was extracted using QIAGEN RNEasy mini kits
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
Description primary human keratinocytes treated with TNF
Data processing Robust Multichip Average (RMA)
 
Submission date Mar 05, 2012
Last update date Mar 07, 2012
Contact name William R Swindell
E-mail(s) [email protected]
Organization name Harvard University
Department Genetics
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL570
Series (1)
GSE36287 Expression data from primary human keratinocytes exposed to cytokines in vitro (IL-4, IL-13, IL-17A, IFN-alpha, IFN-gamma, TNF)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.03209086
1053_at 6.451373854
117_at 4.267610997
121_at 7.066933296
1255_g_at 2.446049055
1294_at 5.969921937
1316_at 4.772415331
1320_at 5.04190286
1405_i_at 5.965830705
1431_at 3.568739402
1438_at 7.116351301
1487_at 8.12569911
1494_f_at 4.955733051
1552256_a_at 8.263775151
1552257_a_at 8.543289229
1552258_at 4.177445671
1552261_at 3.332646109
1552263_at 5.535027937
1552264_a_at 7.273143235
1552266_at 2.512651995

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM886444.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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