|
| Status |
Public on Mar 13, 2012 |
| Title |
E8.5 combined heart mesoderm and head mesoderm/ectoderm 2 |
| Sample type |
RNA |
| |
|
| Source name |
mesoderm/ectoderm from mouse embryos 3-5 somite stage
|
| Organism |
Mus musculus |
| Characteristics |
strain: ARC/s
tissue: mesoderm/ectoderm from heart/head fold
age: E8.5
developmental stage: 4-5 somites
|
| Treatment protocol |
Tissues were separated by mechanical dissection (endoderm 1 and mes/ectoderm 1) or trypsin/pancreatin digestion and mechanical dissection (endoderm 2 and mes/ectoderm 2). 15-20 individual tissue pieces (foregut endoderm, or combined mesoderm/ectoderm from head and heart tissues) were pooled per sample.
|
| Growth protocol |
Mouse embryos were collected at the 4-5 somite stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAGEN RNeasy mini columns following the standard protocol. Concentration and integrity were tested by spectrophotometry and Bioanalyzer analyses.
|
| Label |
biotin
|
| Label protocol |
Biotin labelled using two rounds of amplification with the Affymetrix IVT kit following standard procedures. RNA amplification labelling was performed by the Australian Genome Research Facility.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol. Hybridization was performed by the Australian Genome Research Facility.
|
| Scan protocol |
Affymetrix scanner 3000 7G. Scanning was performed by the Australian Genome Research Facility.
|
| Description |
mes/ectoderm 2
RNA from pooled heart/head mesoderm and ectoderm from 4-5 somite mouse embryos.
|
| Data processing |
Data were processed using tools in GenePattern. CEL files were imported into Expression File Creator and subjected to RMA normalization with quantile normalization.
|
| |
|
| Submission date |
Mar 12, 2012 |
| Last update date |
Mar 13, 2012 |
| Contact name |
David A. F. Loebel |
| E-mail(s) |
[email protected]
|
| Phone |
61-2-88652800
|
| Fax |
61-2-8865 2801
|
| URL |
http://www.cmri.org.au/EMBRYOLOGY/default.aspx
|
| Organization name |
Children's Medical Research Institute
|
| Department |
Embryology Unit
|
| Street address |
214 Hawkesbury Road
|
| City |
Westmead |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE36453 |
Identification of endoderm-specific genes in mouse embryos |
|
