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Status
Public on Nov 03, 2006
Title
90caf2kg
Sample type
RNA
Channel 1 Source name
90caf2_test
Organism
Saccharomyces cerevisiae Characteristics
'drug: caffeine', 'time: 90 min', 'concentration: 20mM'
Extracted molecule
total RNA
Label
Cy5
Channel 2 Source name
90caf2_ctrl
Organism
Saccharomyces cerevisiae Characteristics
'drug: caffeine', 'time: 90 min', 'concentration: 0mM'
Extracted molecule
total RNA
Label
Cy3
Description
Yeast treated at caffeine (20mM) vs non treated. 90 min from the start of treatment.
Data processing
VALUE=log(CH1/CH2)-K, where CH1=max(10,CH1_SIG_MEDIAN-CH1_BKD_MEDIAN), same for CH2. K - is a normalization coefficient K=mean_over_all_spots(log(CH1/CH2))
Submission date
Jan 10, 2006
Last update date
Nov 03, 2006
Contact name
Serguei Sokol
E-mail(s)
[email protected] URL
http://biopuce.insa-toulouse.fr Organization name
INSA
Department
Lab. Biotech. and Bioprocess
Lab
Biopuces
Street address
135 av de Rangueil
City
Toulouse
ZIP/Postal code
31077
Country
France
Platform ID
GPL3250 Series (1)
GSE4049 Time-course studies of three cell wall interfering drugs on gene expression in yeast
Data table header descriptions ID_REF VALUE normalized by total mean log ratio (test over control)
CH1_SIG_MEDIAN median in red channel
CH1_BKD_MEAN background in red channel
CH2_SIG_MEDIAN median in green channel
CH2_BKD_MEAN background in green channel
Data table ID_REF VALUE CH1_SIG_MEDIAN CH1_BKD_MEAN CH2_SIG_MEDIAN CH2_BKD_MEAN 01P09.2
-0.292901
226
64
228
69
01P09.1
-0.340372
202
65
209
68
01P01.2
-0.206232
173
63
168
69
01P01.1
-0.236557
148
65
149
72
01L17.2
-0.0419291
230
65
195
69
01L17.1
-0.0161284
201
72
172
76
01L09.2
-0.201738
3907
91
3525
106
01L09.1
-0.2173
4405
82
4026
92
01L01.2
-0.311593
76
73
76
74
01L01.1
-0.311593
71
71
83
75
01H17.2
1.42301
361
72
131
80
01H17.1
1.46751
389
75
134
81
01H09.2
0.187004
376
78
273
92
01H09.1
0.305182
400
85
269
99
01H01.2
-1.29242
179
95
320
96
01H01.1
-1.06896
195
97
309
100
01D17.2
-0.0801862
8798
109
7004
110
01D17.1
-0.220831
8041
100
7353
101
01D09.2
-0.26203
1573
84
1502
85
01D09.1
0.298314
1499
82
854
84
15488 507 Kbytes .Supplementary data files not provided
