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Status
Public on Nov 03, 2006
Title
90caf2kr
Sample type
RNA
Channel 1 Source name
90caf2_ctrl2
Organism
Saccharomyces cerevisiae Characteristics
'drug: caffeine', 'time: 90 min', 'concentration: 0mM'
Extracted molecule
total RNA
Label
Cy5
Channel 2 Source name
90caf2_test2
Organism
Saccharomyces cerevisiae Characteristics
'drug: caffeine', 'time: 90 min', 'concentration: 20mM'
Extracted molecule
total RNA
Label
Cy3
Description
Yeast treated at caffeine (20mM) vs non treated. 90 min from the start of treatment.
Data processing
VALUE=log(CH2/CH1)-K, where CH1=max(10,CH1_SIG_MEDIAN-CH1_BKD_MEDIAN), same for CH2. K - is a normalization coefficient K=mean_over_all_spots(log(CH2/CH1))
Submission date
Jan 11, 2006
Last update date
Nov 03, 2006
Contact name
Serguei Sokol
E-mail(s)
[email protected] URL
http://biopuce.insa-toulouse.fr Organization name
INSA
Department
Lab. Biotech. and Bioprocess
Lab
Biopuces
Street address
135 av de Rangueil
City
Toulouse
ZIP/Postal code
31077
Country
France
Platform ID
GPL3250 Series (1)
GSE4049 Time-course studies of three cell wall interfering drugs on gene expression in yeast
Data table header descriptions ID_REF VALUE normalized by total mean log ratio (test over control)
CH1_SIG_MEDIAN median in red channel
CH1_BKD_MEAN background in red channel
CH2_SIG_MEDIAN median in green channel
CH2_BKD_MEAN background in green channel
Data table ID_REF VALUE CH1_SIG_MEDIAN CH1_BKD_MEAN CH2_SIG_MEDIAN CH2_BKD_MEAN 01P09.2
0.234772
443
60
315
72
01P09.1
0.169441
380
62
260
71
01P01.2
-0.17436
203
63
128
69
01P01.1
-0.222091
184
72
120
75
01L17.2
0.146462
240
73
181
84
01L17.1
-0.039334
325
70
236
113
01L09.2
-0.382081
5852
69
2090
110
01L09.1
-0.528908
7272
67
2205
75
01L01.2
0.210172
82
61
84
71
01L01.1
0.535594
76
62
81
69
01H17.2
1.57705
181
62
361
72
01H17.1
1.46057
190
60
358
77
01H09.2
-0.0714068
638
60
345
75
01H09.1
-0.0631738
650
64
350
74
01H01.2
-1.14897
389
62
129
77
01H01.1
-0.601239
480
80
192
82
01D17.2
-0.464827
8795
67
2831
80
01D17.1
-0.503896
12168
67
3746
78
01D09.2
-0.576958
677
63
255
82
01D09.1
-0.645431
1206
62
376
75
15488 484 Kbytes .Supplementary data files not provided
