|
| Status |
Public on Apr 25, 2012 |
| Title |
Sfrp5 KO HFD 8 weeks, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
gonadal white adipose tissue_Sfrp5 KO_8 weeks of high-fat diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
genotype/variation: Sfrp5 KO (Q27Stop)
gender: male
age: 12 weeks
diet: 8 wks of high-fat diet
tissue: gonadal white adipose tissue (WAT)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Stat60 extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Nugen WT-Pico Ovation kit protocol from 10 ng total RNA (WT-Pico Ovation RNA Amplification System Manual, 2007, NuGEN). Subsequently cDNA is made double stranded fragmented and end-labeled following manufacturers standard protocol (NuGEN exon module, encore end-labeling module).
|
| |
|
| Hybridization protocol |
Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Gene ST 1.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
MacDougald_10_627_ko3.rma
|
| Data processing |
Data was analyzed using the limma, oligo and affy Bioconductor packages implemented in the R-statistical environment (Affy package v. 1.18 from bioconductor version 2.2). RMA was used to normalize the data and fit log2 transformed expression values (Affy Expression Console version 1.1.2800.19935).
|
| |
|
| Submission date |
Apr 24, 2012 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Ormond MacDougald |
| E-mail(s) |
[email protected]
|
| URL |
http://macdougaldlab.physiology.med.umich.edu/
|
| Organization name |
University of Michigan
|
| Department |
Molecular and Integrative Physiology
|
| Lab |
MacDougald
|
| Street address |
6458 Brehm Center, 1000 Wall Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48105 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE37514 |
Effect of ablation of Sfrp5 on gene expression in gonadal white adipose tissue |
|
