|
| Status |
Public on Aug 16, 2012 |
| Title |
Treated_8637 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse testis tissue
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: C57/129
agent: JQ1
tissue: Testis
|
| Treatment protocol |
Mice were treated with JQ1 (50 mg/kg) or vehicle daily for 6 weeks (6-12 weeks of age).
|
| Growth protocol |
Mice were housed in a light-controlled environment, and were fed standard chow ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent. RNA quality was checked using a RNA Nano chips on the Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
The total RNA was amplified and purified using Illumina TotalPrep RNA Amplification Kit (Ambion, Cat# IL1791) following kit instructions. Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
JO1 sample 3
Sample name: 8637
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R. Technical replicates for Control sample 1 were averaged after normalization for subsequent analyses.
|
| |
|
| Submission date |
May 10, 2012 |
| Last update date |
Aug 16, 2012 |
| Contact name |
Qinglei Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Texas A&M University
|
| Street address |
4458 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE37894 |
Testis Gene Expression Changes after JQ1 treatment |
|
