|
| Status |
Public on Aug 13, 2012 |
| Title |
Lgr5-GFP_high adenoma vs Lgr5-GFP_low adenoma array_2 dyeswap |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
FACS sorted intestinal adenoma cells from induced Apc_fl/fl-Lgr5-EGFP-ires-CreERT2 mice
|
| Organism |
Mus musculus |
| Characteristics |
gfp level: low
strain: C57BL/6
|
| Growth protocol |
RNA was isolated from two FACS sorted cell populations of intestinal adenomas from induced Apc_fl/fl-Lgr5_EGFP_ires_CreERT2 mice : adenoma GFP-high cells and adenoma GFP-low cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
300 ng of total RNA was labeled using low RNA Input Linear Amp kit (Agilent Technologies, Pato Alto, CA, USA).
|
| |
|
| Channel 2 |
| Source name |
FACS sorted intestinal adenoma cells from induced Apc_fl/fl-Lgr5-EGFP-ires-CreERT2 mice
|
| Organism |
Mus musculus |
| Characteristics |
gfp level: high
strain: C57BL/6
|
| Growth protocol |
RNA was isolated from two FACS sorted cell populations of intestinal adenomas from induced Apc_fl/fl-Lgr5_EGFP_ires_CreERT2 mice : adenoma GFP-high cells and adenoma GFP-low cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
300 ng of total RNA was labeled using low RNA Input Linear Amp kit (Agilent Technologies, Pato Alto, CA, USA).
|
| |
|
| |
| Hybridization protocol |
according to Agilent guidelines
|
| Scan protocol |
according to Agilent guidelines
|
| Data processing |
Microarray signal and background information were retrieved using featuere extraction (V.9.5.3, Agilent Technologies).
|
| |
|
| Submission date |
May 11, 2012 |
| Last update date |
Aug 13, 2012 |
| Contact name |
Daniel E. Stange |
| E-mail(s) |
[email protected]
|
| Organization name |
University Clinic Dresden
|
| Street address |
Fetscherstr. 74
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE37926 |
Gene expression profiling of Lgr5 positive cells within intestinal adenomas |
| GSE37929 |
Expression profiling of Lgr5 positive adenoma cells. |
|
