|
| Status |
Public on May 22, 2012 |
| Title |
USC_ChipSeq_HeLa-S3_AP-2gamma_std |
| Sample type |
SRA |
| |
|
| Source name |
HeLa-S3
|
| Organism |
Homo sapiens |
| Characteristics |
lab: USC
lab description: Farnham - University of Southern California
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: HeLa-S3
cell organism: human
cell description: cervical carcinoma
cell karyotype: cancer
cell lineage: ectoderm
cell sex: F
treatment: None
treatment description: No special treatment or protocol applies
antibody: AP-2gamma
antibody antibodydescription: Rabbit polyclonal - AP-2Y (H-77) is a rabbit polyclonal antibody raised against amino acids 145-221 mapping with in an internal region of AP-2Y of human origin. Antibody Target: AP-2gamma
antibody targetdescription: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-8977
control: std
control description: Standard input signal for most experiments.
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000612
labversion: Fragmented using Bioruptor, precipitated with StaphA
replicate: 1
softwareversion: PeakSeq1.0 (fdr 0.001)
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
None
|
| Growth protocol |
HeLa-S3_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
[email protected]
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX150450 |
| BioSample |
SAMN01000911 |
| Named Annotation |
GSM935370_hg19_wgEncodeSydhTfbsHelas3Ap2gammaStdSig.bigWig |
