|
| Status |
Public on May 22, 2012 |
| Title |
Stanford_ChipSeq_HeLa-S3_IRF3_IgG-rab |
| Sample type |
SRA |
| |
|
| Source name |
HeLa-S3
|
| Organism |
Homo sapiens |
| Characteristics |
lab: Stanford
lab description: Snyder - Stanford University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: HeLa-S3
cell organism: human
cell description: cervical carcinoma
cell karyotype: cancer
cell lineage: ectoderm
cell sex: F
treatment: None
treatment description: No special treatment or protocol applies
antibody: IRF3
antibody antibodydescription: Rabbit polyclonal; epitope corresponding to amino acids 1-425 of IRF-3 of human origin. Antibody Target: IRF3
antibody targetdescription: Mediates interferon-stimulated response element (ISRE) promoter activation. DsRNA leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-9082
control: IgG-rab
control description: Input signal from Normal Rabbit IgG ChIP-seq.
control: IgG-rab
control description: Input signal from Normal Rabbit IgG ChIP-seq.
controlid: wgEncodeEH000744
replicate: 1
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
None
|
| Growth protocol |
HeLa-S3_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
[email protected]
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX150649 |
| BioSample |
SAMN01001109 |
| Named Annotation |
GSM935570_hg19_wgEncodeSydhTfbsHelas3Irf3IggrabSig.bigWig |
