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Sample GSM943794 Query DataSets for GSM943794
Status Public on Jan 11, 2014
Title 4F_Day0, biological rep1
Sample type RNA
 
Source name MEFs, day 0
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblasts (MEFs)
retrovirus: none
time: day 0
Treatment protocol MEFs were infected with retroviruses encoding the four transcription factors (Oct4, Sox2, Klf4 and c-Myc), three transcription factors (Oct4, Sox2 and Klf4) or GFP (control) at day 0.
Growth protocol MEFs were maintained in Dulbecco's Modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS).
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the Qiagen RNeasy kit according to the manufacturer's protocol. On-column DNase digestion was performed.
Label biotin
Label protocol Biotinylated cRNAs were prepared from 120 ng (rep1) or 300 ng (the others) total RNAs according to the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay manual.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol with Affymetrix Scanner 3000 7G
Description 4F_Day0-1
Gene expression data from MEFs, day 0.
Data processing The probeset signals were calculated using Agilent GeneSpring software.
 
Submission date Jun 05, 2012
Last update date Sep 08, 2016
Contact name Miki Ebisuya
E-mail(s) [email protected]
Organization name EMBL
Department EMBL Barcelona
Lab Lab for Synthetic Developmental Biology
Street address PRBB Building, C/ Dr. Aiguader, 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platform ID GPL6246
Series (1)
GSE38509 Gene expression profiling in the reprogramming process

Data table header descriptions
ID_REF
VALUE Log2 RMA

Data table
ID_REF VALUE
10344614 6.0612917
10344616 4.3713603
10344618 4.412663
10344620 4.624053
10344622 6.9041877
10344624 8.896498
10344633 9.8768425
10344637 8.636042
10344653 4.824603
10344658 7.65462
10344674 4.880621
10344679 5.0883064
10344705 5.719192
10344707 6.7063437
10344713 10.495422
10344715 4.612425
10344717 5.531423
10344719 6.2666755
10344721 4.348972
10344723 8.719418

Total number of rows: 28853

Table truncated, full table size 518 Kbytes.




Supplementary file Size Download File type/resource
GSM943794_4F_Day0-1.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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