|
Status |
Public on Jan 11, 2014 |
Title |
4F_Day0, biological rep1 |
Sample type |
RNA |
|
|
Source name |
MEFs, day 0
|
Organism |
Mus musculus |
Characteristics |
cell type: mouse embryonic fibroblasts (MEFs) retrovirus: none time: day 0
|
Treatment protocol |
MEFs were infected with retroviruses encoding the four transcription factors (Oct4, Sox2, Klf4 and c-Myc), three transcription factors (Oct4, Sox2 and Klf4) or GFP (control) at day 0.
|
Growth protocol |
MEFs were maintained in Dulbecco's Modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using the Qiagen RNeasy kit according to the manufacturer's protocol. On-column DNase digestion was performed.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNAs were prepared from 120 ng (rep1) or 300 ng (the others) total RNAs according to the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay manual.
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol with Affymetrix Scanner 3000 7G
|
Description |
4F_Day0-1 Gene expression data from MEFs, day 0.
|
Data processing |
The probeset signals were calculated using Agilent GeneSpring software.
|
|
|
Submission date |
Jun 05, 2012 |
Last update date |
Sep 08, 2016 |
Contact name |
Miki Ebisuya |
E-mail(s) |
[email protected]
|
Organization name |
EMBL
|
Department |
EMBL Barcelona
|
Lab |
Lab for Synthetic Developmental Biology
|
Street address |
PRBB Building, C/ Dr. Aiguader, 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE38509 |
Gene expression profiling in the reprogramming process |
|