|
| Status |
Public on Sep 15, 2012 |
| Title |
0-24 Hrs control embryos, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
0-24 hour old embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dechorionated embryo
age: 0-24 hour old
genotype/variation: control (Gal 4 expressing animals)
|
| Treatment protocol |
Embryos were dechorionated with 100% bleach, and washed with D.I. water several times. Washed and drained embryos were placed in the Trizol solution (Invitrogen).
|
| Growth protocol |
For both genotypes (Control and dMyc+) 0-24 hours embryos were collected on a grape juice agar at 25C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol solution (Invitrogen), according to manufacturer’s recommendation.
|
| Label |
biotin
|
| Label protocol |
3” IVT Express kit was used. Done by Expression Analysis (Durham, NC, USA)
|
| |
|
| Hybridization protocol |
Affymetrix hybridization protocol. Done by Expression Analysis (Durham, NC, USA)
|
| Scan protocol |
Affymetrix GeneChip Scannerl. Done by Expression Analysis (Durham, NC, USA)
|
| Description |
Gene expression data from embryos between 0 t0 24 hours old
|
| Data processing |
The data were analyzed using Affymetrix default analysis settings and global scaling as normalization method. Done by Expression Analysis (Durham, NC, USA)
|
| |
|
| Submission date |
Jun 06, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kaveh Daneshvar |
| E-mail(s) |
[email protected]
|
| Organization name |
University of North Carolina at Charlotte
|
| Department |
Biology
|
| Street address |
9201 University City Blvd.
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28223 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE38529 |
Expression data from Drosophila embryos, control vs. dMyc+ |
|
| Relations |
| Reanalyzed by |
GSE119084 |
