|
| Status |
Public on Jul 16, 2012 |
| Title |
PN21 mouse retina, biological rep1 Edn2-/- |
| Sample type |
RNA |
| |
|
| Source name |
PN21 mouse retina, biological rep1 Edn2-/-
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole retina
age: postnatal day 21
genotype: Edn2-/-
genetic background: 50% C57BL\6J, 50% 129Sv/Ev (Taconic)
|
| Treatment protocol |
Eyes were dissected in ice-cold 1X PBS by removing the corneas and peeling off the retina from the underlying sclera and choroid
|
| Growth protocol |
mice of the appropriate genotype were sacrificed at PN21 for extraction of retinal RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from two retinas (one mouse) was homogenized in GIT buffer and isolated by cesium chloride density gradient centrifugation.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Afymetrix protocol from 2.5 ug total RNA (Affymetrix one-cycle kit and IVT kit)
|
| |
|
| Hybridization protocol |
EukGE-WS2v5. 15 ug of cRNA were hybridized.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from retinas at PN21
|
| Data processing |
The data were analyzed using the Robust Multi-array Averaging algorithm (RMA) with Affymetric GeneChip Operating Software (GCOS). The scaling target signal was set to 150.
|
| |
|
| Submission date |
Jun 18, 2012 |
| Last update date |
Jul 16, 2012 |
| Contact name |
Alexa Natalie Bramall |
| E-mail(s) |
[email protected]
|
| Organization name |
Hospital for Sick Children
|
| Street address |
101 College St.
|
| City |
Toronto |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE38797 |
Expression values from wild-type retinas and retinas with a mutant Rhodopsin transgene (Tg(RHO P347S)) with and without Endothelin-2 (EDN2) |
|
