|
| Status |
Public on Sep 04, 2012 |
| Title |
SIN3A siRNA, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Calu-3_SIN3A siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Calu-3
cell type: airway epithelia cells
treated with: SIN3A siRNA
|
| Treatment protocol |
Cells were transfected with oligonucleotides using lipofectamine RNAiMAX.
|
| Growth protocol |
Calu-3 cells were grown in complete mendia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZOL and manufacturer's recommended protocol was followed to extract RNA from primary cultures
|
| Label |
biotin
|
| Label protocol |
Total RNA (5 μg) is processed using the Affymetrix GeneChip one-cycle target labeling kit (Affymetrix) according to the manufacturer’s recommended protocols.
|
| |
|
| Hybridization protocol |
The resultant biotinylated cRNA is fragmented and then hybridized to The GeneChip® Porcine Genome Array. The GeneChip® Porcine Genome Array provides comprehensive coverage of the Sus scrofa transcriptome. The array contains 23,937 probe sets that interrogate approximately 23,256 transcripts from 20,201 S. scrofa genes.
|
| Scan protocol |
The arrays are washed, stained, and scanned using the Affymetrix Model 450 Fluidics Station and Affymetrix Model 3000 scanner using the manufacturer’s recommended protocols, and the data collected using the GeneChip Operating Software (GCOS), v.1.4.
|
| Description |
SIN3AsiRNA-2
|
| Data processing |
Data processing and analysis was performed using the Partek Genomics Suite v.6.3 (RMA normalization).
|
| |
|
| Submission date |
Jun 26, 2012 |
| Last update date |
Sep 04, 2012 |
| Contact name |
Shyam Ramachandran |
| E-mail(s) |
[email protected]
|
| Phone |
3193356933
|
| Organization name |
Univ of Iowa
|
| Department |
Pediatrics
|
| Lab |
McCray Lab
|
| Street address |
240G EMRB
|
| City |
Iowa City |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE38956 |
A microRNA network regulates expression and biosynthesis of CFTR and CFTR-ΔF508. |
|
