|
| Status |
Public on Nov 27, 2013 |
| Title |
PR48 cells, technical replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Kasumi-1 cells, dasatinib-treated for 12 weeks, treatment discontinued for 1 week
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Kasumi-1
|
| Treatment protocol |
R48 cells were derived from Kasumi-1 (K) cells by continuiously co-culture with dasatinib for 12 weeks. Treatment was initiated at 12 nM for 4 weeks and subsequently increased to 24 nM and 48 nM after 4 weeks, respectively. PR48 cells were derived from R48 cells by withdrawal of dasatinib and cessation of treatment for 1 week.
|
| Growth protocol |
Kasumi-1 cells were grown at 0.5 x 10^6 cells/ml Gibco RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 20% fetal calf serum in a humidified incubator at 37°C with 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total mRNA was performed using Trizol (Invitrogen) according to manufacturer's intructions.
|
| Label |
biotin
|
| Label protocol |
Whole Transcript (WT) Sense Target Labeling Assay
|
| |
|
| Hybridization protocol |
according to Affymetrix Whole Transcript (WT) Sense Target Labeling Assay
|
| Scan protocol |
according to Affymetrix protocols using a GeneChip 3000 Scanner
|
| Data processing |
Affymetrix® Expression Console™ Software for normalization, BRB Array Tools for statistical analysis
|
| |
|
| Submission date |
Jul 03, 2012 |
| Last update date |
Nov 28, 2013 |
| Contact name |
Karlheinz Holzmann |
| E-mail(s) |
[email protected]
|
| Organization name |
Ulm University
|
| Department |
Medical Faculty
|
| Lab |
Genomics Core Facility
|
| Street address |
Helmholtzstreet 8/1
|
| City |
Ulm |
| ZIP/Postal code |
D-89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE39073 |
Expression data from a reversible dasatinib-resistant state in long-term dasatinib-treated c-KIT-mutated Kasumi-1 cell line |
|
