|
| Status |
Public on Aug 01, 2012 |
| Title |
healthy subject-03 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC
sample type: healthy
Sex: male
age: 35
|
| Treatment protocol |
All the cancer patients received first line chemotherapy with CDDP (mean dose 70 mg/m2) on day 1 plus GEM (GEMZAR®; EliLilly and Company, Indianapolis, IN; mean dose 1000 mg/m2) on day 1, 8, and 15 of a 28-day cycle. Treatment was planned for a maximum of six cycles (median 4, range 1-6) unless discontinued because of progressive disease, stable disease, unacceptable toxicity, or death.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 13, 2012 |
| Last update date |
Aug 01, 2012 |
| Contact name |
yungche chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Kaohsiung Chang Gung Memorial Hospital
|
| Street address |
123, Ta-Pei Rd. Niao-Sung Dist.
|
| City |
Kaohsiung |
| ZIP/Postal code |
833 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE39345 |
Microarray gene expression profiles of peripheral blood mononuclear cells in patients with advanced stage non-small cell lung cancer treated with first line combination chemotherapy |
|
