|
| Status |
Public on Sep 15, 2012 |
| Title |
Wild-type MEFs 2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type MEFs
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57B/L6
genotype: Wild-type
cell type: Cultured T antigen transformed Mouse Embrionic Fibroblasts
|
| Growth protocol |
Cultured in DMEM with NEAA, Pencillin Streptomycin, Glutamax
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted using Trizol (Invitrogen) according to manufacturer's instruction
|
| Label |
Biotin
|
| Label protocol |
Labelled using Ambion WT Expression Kit and Affymetrix Hybridization, Wash and Stain Kit
|
| |
|
| Hybridization protocol |
Hybridized using Affymetrix Hybridization, Wash and Stain Kit
|
| Scan protocol |
GCS3000 using AGCC 3
|
| Description |
Wild-type MEFs
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jul 19, 2012 |
| Last update date |
Sep 15, 2012 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE39492 |
Ascorbic acid depletion and scorbutic extracellular matrix in mice deficient in endoplasmic reticulum thiol oxidases |
|
