|
| Status |
Public on Jul 31, 2012 |
| Title |
T47D control ETOH (vehicle) |
| Sample type |
RNA |
| |
|
| Source name |
T47D cells, treated for 3 hours with 0.1% Ethanol
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T47D
protocol: ethanol treated cells (vehicle) 3h
|
| Treatment protocol |
T47D cells were incubated with Estradiol or ERα17p for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS
|
| Growth protocol |
The human breast cancer cell line T47D was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
|
| |
|
| Hybridization protocol |
RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
|
| Scan protocol |
Signals were detected by an Affymetrix microarray chip reader.
|
| Data processing |
Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
Summarization Algorithm: RMA
Technology: Affymetrix.GeneChip.HG-U133_Plus_2
Normalization: Quantile
Baseline Transformation: median of all samples
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Jul 31, 2012 |
| Contact name |
George Notas |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Crete
|
| Department |
Medicine
|
| Lab |
Experimental Endocrinology
|
| Street address |
PO BOX 2208
|
| City |
Heraklion |
| ZIP/Postal code |
71003 |
| Country |
Greece |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE39720 |
Time-course effect of estradiol and ERa17p on Early Gene expression in T47D cells |
| GSE39721 |
Time-course effect of estradiol and ERa17p on Early Gene expression in human breast cancer cell lines |
|
