|
| Status |
Public on Mar 05, 2014 |
| Title |
plant_treated_with_combined_stress_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
20d of above-ground parts, Arabidopsis thaliana, 150mM salt (16hrs), 200mM mannitol (16hrs), 35°C heat (4hrs), replicate2
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
tissue: above-ground parts
age: 20d
genotype: wild type
treatment: 150mM salt, 16hrs + 200mM mannitol, 16hrs + 35°C heat, 4hrs
|
| Treatment protocol |
Soil-grown plants were treated with 150mM, 300mM of salt and/or 200mM, 400mM of mannitol for 16 hrs by feeding the solution and/or 35°C heat for 4 hrs by placing plants in incubator.
|
| Growth protocol |
All plants were grown in MS (1% sucrose) agar media for first 10 days and in soil for next 10 days in 16hr light / 8hr dark cycle in 23 Celsius degree before stress treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy3
|
| Label protocol |
1 µg of total RNA was used for labeling procedure using QickAmp Labeling kit (One-color) (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized using Gene Expression Hybridization kit (Agilent) and washed by Gene Expression wash pack (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
|
| Description |
Gene expression of plants treated with 150mM salt, 200mM mannitol and 35°C heat, simultaneously
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction. After that, signal of each probe was divided by median value of filter-passed probes.
|
| |
|
| Submission date |
Aug 08, 2012 |
| Last update date |
Mar 05, 2014 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE39956 |
Combined stress treatment of high salinity, osmotic pressure and heat to Arabidopsis thaliana |
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