|
| Status |
Public on Jun 01, 2006 |
| Title |
gdxmale_day7_P.chabaudi_infected_rep02 |
| Sample type |
RNA |
| |
|
| Source name |
WBCs from spleen
|
| Organism |
Mus musculus |
| Characteristics |
'strain:C57BL/6', 'sex:male', 'treatment:gdx', 'age:8-10wks', 'tissue: WBC from spleen', 'condition: infected 7 days'
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol extraction followed by qiagen RNeasy total RNA cleanup protocol
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures using affy GCS3000
|
| Description |
Intensity of hybridization for each probe pair was computed by GCOS 1.2 software (Affymetrix). (For more detailed methods, please refer to the website of the Malaria Research Institute Gene Array Core Facility (MRI-GACF) at the Johns Hopkins Bloomberg School of Public Health http://jhmmi.jhsph.edu)
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Mar 01, 2006 |
| Last update date |
Mar 02, 2006 |
| Contact name |
Sabra Lynn Klein |
| E-mail(s) |
[email protected]
|
| Phone |
(410) 955-8898
|
| Fax |
(410) 955-0105
|
| Organization name |
Johns Hopkins Bloomberg School of Public Health
|
| Department |
Molecular Microbiology and Immunology
|
| Lab |
Lab of Sabra Klein
|
| Street address |
615 North Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE4324 |
Sex Differences in Response to Plasmodium chabaudi Infection: Involvement of Gonadal Steroids |
|
