tissue: brain, left frontal temporal lobe histology: GBM tumor number (as appears in figure 3): 1675 age: 61 gender: MALE grade: 4 living: DECEASED survival time: 683 survival cluster: SC2 hc_74_samples: HC2B hc_85_samples: HC2B array: 133A
Extracted molecule
total RNA
Extraction protocol
RNA extraction was performed as described previously (Mischel PS, Shai R, Shi T, et al Identification of molecular subtypes of glioblastoma by gene expression profiling. Oncogene, 2003;22:2361-73) and analyzed on both a spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Label protocol
Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A and HG-U133B arrays (Affymetrix, Santa Clara, CA).
