LOCUS NM_123774 1815 bp mRNA linear PLN 20-OCT-2022
DEFINITION Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.
ACCESSION NM_123774
VERSION NM_123774.4
DBLINK BioProject: PRJNA116
BioSample: SAMN03081427
KEYWORDS RefSeq.
SOURCE Arabidopsis thaliana (thale cress)
ORGANISM Arabidopsis thaliana
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
Camelineae; Arabidopsis.
REFERENCE 1 (bases 1 to 1815)
AUTHORS Tabata,S., Kaneko,T., Nakamura,Y., Kotani,H., Kato,T., Asamizu,E.,
Miyajima,N., Sasamoto,S., Kimura,T., Hosouchi,T., Kawashima,K.,
Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A., Nakayama,S.,
Nakazaki,N., Naruo,K., Okumura,S., Shinpo,S., Takeuchi,C., Wada,T.,
Watanabe,A., Yamada,M., Yasuda,M., Sato,S., de la Bastide,M.,
Huang,E., Spiegel,L., Gnoj,L., O'Shaughnessy,A., Preston,R.,
Habermann,K., Murray,J., Johnson,D., Rohlfing,T., Nelson,J.,
Stoneking,T., Pepin,K., Spieth,J., Sekhon,M., Armstrong,J.,
Becker,M., Belter,E., Cordum,H., Cordes,M., Courtney,L.,
Courtney,W., Dante,M., Du,H., Edwards,J., Fryman,J., Haakensen,B.,
Lamar,E., Latreille,P., Leonard,S., Meyer,R., Mulvaney,E.,
Ozersky,P., Riley,A., Strowmatt,C., Wagner-McPherson,C., Wollam,A.,
Yoakum,M., Bell,M., Dedhia,N., Parnell,L., Shah,R., Rodriguez,M.,
See,L.H., Vil,D., Baker,J., Kirchoff,K., Toth,K., King,L.,
Bahret,A., Miller,B., Marra,M., Martienssen,R., McCombie,W.R.,
Wilson,R.K., Murphy,G., Bancroft,I., Volckaert,G., Wambutt,R.,
Dusterhoft,A., Stiekema,W., Pohl,T., Entian,K.D., Terryn,N.,
Hartley,N., Bent,E., Johnson,S., Langham,S.A., McCullagh,B.,
Robben,J., Grymonprez,B., Zimmermann,W., Ramsperger,U., Wedler,H.,
Balke,K., Wedler,E., Peters,S., van Staveren,M., Dirkse,W.,
Mooijman,P., Lankhorst,R.K., Weitzenegger,T., Bothe,G., Rose,M.,
Hauf,J., Berneiser,S., Hempel,S., Feldpausch,M., Lamberth,S.,
Villarroel,R., Gielen,J., Ardiles,W., Bents,O., Lemcke,K.,
Kolesov,G., Mayer,K., Rudd,S., Schoof,H., Schueller,C.,
Zaccaria,P., Mewes,H.W., Bevan,M. and Fransz,P.
CONSRTM Kazusa DNA Research Institute; Cold Spring Harbor and Washington
University in St Louis Sequencing Consortium; European Union
Arabidopsis Genome Sequencing Consortium
TITLE Sequence and analysis of chromosome 5 of the plant Arabidopsis
thaliana
JOURNAL Nature 408 (6814), 823-826 (2000)
PUBMED 11130714
REFERENCE 2 (bases 1 to 1815)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (19-OCT-2022) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 1815)
AUTHORS Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M.,
Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R.,
Vaughn,M. and Town,C.D.
TITLE Direct Submission
JOURNAL Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute,
9704 Medical Center Dr, Rockville, MD 20850, USA
REMARK Protein update by submitter
REFERENCE 4 (bases 1 to 1815)
AUTHORS Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E.
CONSRTM TAIR
TITLE Direct Submission
JOURNAL Submitted (18-FEB-2011) Department of Plant Biology, Carnegie
Institution, 260 Panama Street, Stanford, CA, USA
COMMENT REVIEWED REFSEQ: This record has been curated by TAIR and Araport.
This record is derived from an annotated genomic sequence
(NC_003076).
On Sep 12, 2016 this sequence version replaced NM_123774.3.
FEATURES Location/Qualifiers
source 1..1815
/organism="Arabidopsis thaliana"
/mol_type="mRNA"
/db_xref="taxon:3702"
/chromosome="5"
/ecotype="Columbia"
gene 1..1815
/gene="CAD1"
/locus_tag="AT5G44070"
/gene_synonym="ARA8; ARABIDOPSIS THALIANA PHYTOCHELATIN
SYNTHASE 1; ATPCS1; CADMIUM SENSITIVE 1; MRH10.18;
MRH10_18; PCS1; PHYTOCHELATIN SYNTHASE; PHYTOCHELATIN
SYNTHASE 1"
/note="Phytochelatin synthase gene confers tolerance to
cadmium ions. Catalyzes phytochelatin (PC) synthesis from
glutathione (GSH) in the presence of Cd2+, Zn2+, Cu2+ and
Fe3+, but not by Co2+ or Ni2+."
/db_xref="Araport:AT5G44070"
/db_xref="GeneID:834430"
/db_xref="TAIR:AT5G44070"
CDS 171..1628
/gene="CAD1"
/locus_tag="AT5G44070"
/gene_synonym="ARA8; ARABIDOPSIS THALIANA PHYTOCHELATIN
SYNTHASE 1; ATPCS1; CADMIUM SENSITIVE 1; MRH10.18;
MRH10_18; PCS1; PHYTOCHELATIN SYNTHASE; PHYTOCHELATIN
SYNTHASE 1"
/inference="Similar to RNA sequence,
EST:INSD:AV526117.1,INSD:AV562203.1,INSD:EL009460.1,
INSD:ES195192.1,INSD:EG470564.1,INSD:EL331779.1,
INSD:ES172251.1,INSD:DR347043.1,INSD:ES096721.1,
INSD:DR347035.1,INSD:EL058944.1,INSD:AV821622.1,
INSD:AV538749.1,INSD:EL262415.1,INSD:BP576052.1,
INSD:EL188746.1,INSD:BP581751.1,INSD:DR347042.1,
INSD:EL248440.1,INSD:AV793727.1,INSD:EL006564.1,
INSD:EL291851.1,INSD:EL263238.1,INSD:EH836707.1,
INSD:BP563228.1,INSD:BP632687.1,INSD:EG470635.1,
INSD:AV526107.1,INSD:EG470566.1,INSD:BP868419.1,
INSD:EL149933.1,INSD:BP622382.1,INSD:BP606352.1,
INSD:BP589967.1,INSD:DR347040.1,INSD:BP669465.1,
INSD:AI995885.1,INSD:BP668203.1,INSD:DR347048.1,
INSD:DR347047.1,INSD:EH821481.1,INSD:BP866345.1,
INSD:DR347041.1,INSD:EL047588.1,INSD:AV551060.1,
INSD:BP561535.1,INSD:BP664697.1,INSD:ES194183.1,
INSD:EH883676.1,INSD:BP572753.1,INSD:EH799722.1,
INSD:AV520044.1,INSD:EL198815.1,INSD:EG470636.1,
INSD:EH876962.1,INSD:DR347050.1,INSD:DR347049.1,
INSD:BE662956.1,INSD:BU636703.1,INSD:EH838089.1,
INSD:AV782133.1,INSD:EH858210.1,INSD:DR381517.1,
INSD:DR347044.1,INSD:AV536560.1,INSD:BP666345.1,
INSD:BP778309.1,INSD:EL232794.1,INSD:EH885925.1,
INSD:EL008813.1,INSD:EL275935.1,INSD:BP801361.1,
INSD:ES203481.1,INSD:BP790452.1,INSD:CB263626.1,
INSD:EL010804.1,INSD:BP794880.1,INSD:DR347037.1,
INSD:DR347036.1,INSD:BP868498.1,INSD:EL101328.1,
INSD:DR347045.1,INSD:DR338861.1,INSD:EH852469.1,
INSD:DR347034.1,INSD:EH875306.1,INSD:BP595762.1,
INSD:DR347038.1,INSD:DR374388.1,INSD:BP862356.1,
INSD:DR347039.1,INSD:BP858088.1"
/inference="similar to RNA sequence,
mRNA:INSD:AF085230.1,INSD:AY048257.1,INSD:AY079384.1,
INSD:EU218538.1,INSD:BX830828.1,INSD:AF135155.1,
INSD:BX833258.1,INSD:AF461180.1,INSD:AF162689.1,
INSD:AF093753.1,INSD:AY039951.1"
/note="CADMIUM SENSITIVE 1 (CAD1); FUNCTIONS IN: cadmium
ion binding, copper ion binding, glutathione
gamma-glutamylcysteinyltransferase activity; INVOLVED IN:
in 6 processes; LOCATED IN: cytosol; EXPRESSED IN: 24
plant structures; EXPRESSED DURING: 13 growth stages;
CONTAINS InterPro DOMAIN/s: Phytochelatin synthase,
C-terminal (InterPro:IPR015407), Phytochelatin synthase
(InterPro:IPR007719); BEST Arabidopsis thaliana protein
match is: phytochelatin synthase 2 (TAIR:AT1G03980.1); Has
1807 Blast hits to 1807 proteins in 277 species: Archae -
0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385;
Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink)."
/codon_start=1
/product="phytochelatin synthase 1 (PCS1)"
/protein_id="NP_199220.1"
/db_xref="GeneID:834430"
/db_xref="TAIR:AT5G44070"
/db_xref="Araport:AT5G44070"
/translation="MAMASLYRRSLPSPPAIDFSSAEGKLIFNEALQKGTMEGFFRLI
SYFQTQSEPAYCGLASLSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVVKEKGI
SFGKVVCLAHCSGAKVEAFRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQTGT
GHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLISR
PHREPGLLYTLSCKDESWIEIAKYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQ
FIRWVAEIRITEDSNQNLSAEEKSRLKLKQLVLKEVHETELFKHINKFLSTVGYEDSL
TYAAAKACCQGAEILSGSPSKEFCCRETCVKCIKGPDDSEGTVVTGVVVRDGNEQKVD
LLVPSTQTECECGPEATYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQLISMASL
PTLLQEEVLHLRRQLQLLKRCQENKEEDDLAAPAY"
ORIGIN
1 aataattgag tgagtctgta tattaaatac gcaaatcatc atcttcccaa tttcgaatct
61 cacacaccga aagttatttt cgaatccact aacgaatctt ccacagcaaa cacttttgtg
121 ttcctctgta atttctcagt atatatagat accaaaacaa gcagtgaaaa atggctatgg
181 cgagtttata tcggcgatct cttccttctc ctccggccat tgacttttct tccgccgaag
241 gcaagctaat cttcaatgaa gcgcttcaga aaggaactat ggaaggattt ttcaggttga
301 tttcgtattt tcagacacaa tccgaacctg cgtattgtgg tttggctagt ctctcagtgg
361 tgttgaatgc tctttctatc gatcctggac gtaaatggaa agggccttgg aggtggtttg
421 atgaatcaat gttggattgc tgcgaacctc tggaagtagt gaaggaaaaa ggcatttcat
481 ttggaaaagt tgtctgtttg gctcattgtt caggagcaaa agttgaggct ttccgtacaa
541 gtcagagcac cattgatgat ttccgcaaat ttgtcgtcaa atgcacgagt tctgagaatt
601 gtcatatgat ctcaacatat caccgaggtg tatttaagca gactgggact ggtcactttt
661 cacctattgg tggctataat gctgagagag atatggcttt gattcttgat gttgctcgtt
721 tcaagtatcc ccctcactgg gttcctctta aacttctttg ggaagccatg gacagtattg
781 atcagtcaac agggaaacgt agagggttca tgctcatatc tagaccacac agagaacccg
841 gattgctcta tactctgagc tgcaaggatg aaagctggat cgaaatagcc aagtatttga
901 aggaagatgt tcctcgtctt gtaagttcac agcatgtaga ttctgtggag aaaatcatat
961 cagttgtgtt caagtcactt ccatcaaatt tcaaccaatt catcagatgg gtggctgaga
1021 tccgaattac agaggactca aaccaaaatc tcagcgcaga ggagaagtct aggctgaaac
1081 taaagcaatt ggtgctgaag gaagtgcacg aaactgaact gttcaaacac atcaataagt
1141 tcttatccac agtgggttat gaagacagtc tgacttatgc tgctgcaaag gcttgttgcc
1201 aaggagctga aatcttatcc ggaagcccat caaaagagtt ttgttgtcgg gaaacttgcg
1261 tgaaatgcat caaaggtcct gatgactctg aaggcacggt ggtgactgga gttgtggtgc
1321 gtgatgggaa tgaacaaaag gttgatctgt tagtgccatc gacgcaaact gagtgtgaat
1381 gtggtcctga agcaacttat ccagcaggaa acgatgtgtt cactgcactt ctattggctt
1441 tacctccaca gacatggtca gggatcaaag accaagctct tatgcatgaa atgaagcagc
1501 tcatttccat ggcttccctc ccaactttgc ttcaagaaga ggtattgcat cttcgacggc
1561 aacttcagct gctaaaacga tgccaagaga acaaggaaga ggatgatctc gctgctcctg
1621 cctattagtt cattgtccca aatcctctct cttccccatt tgaatcccac gttctcaaca
1681 cttaattgtt agaaagtctc tttattctct gtacgattca aactctattt gcaatgagag
1741 atatatgtaa cttgcattct ataaattgtt aatcacaata agttaagaat cctagaatca
1801 tatttcatca cactc
//