LOCUS NM_016652 4406 bp mRNA linear PRI 21-SEP-2024
DEFINITION Homo sapiens crooked neck pre-mRNA splicing factor 1 (CRNKL1),
transcript variant 1, mRNA.
ACCESSION NM_016652
VERSION NM_016652.6
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4406)
AUTHORS Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
Harper,J.W. and Gygi,S.P.
TITLE Dual proteome-scale networks reveal cell-specific remodeling of the
human interactome
JOURNAL Cell 184 (11), 3022-3040 (2021)
PUBMED 33961781
REFERENCE 2 (bases 1 to 4406)
AUTHORS Xiao,H., Wyler,E., Milek,M., Grewe,B., Kirchner,P., Ekici,A.,
Silva,A.B.O.V., Jungnickl,D., Full,F., Thomas,M., Landthaler,M.,
Ensser,A. and Uberla,K.
TITLE CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1
and Cellular mRNAs
JOURNAL mBio 12 (1), e02525-20 (2021)
PUBMED 33468685
REMARK GeneRIF: CRNKL1 Is a Highly Selective Regulator of Intron-Retaining
HIV-1 and Cellular mRNAs.
Publication Status: Online-Only
REFERENCE 3 (bases 1 to 4406)
AUTHORS Bertram,K., Agafonov,D.E., Liu,W.T., Dybkov,O., Will,C.L.,
Hartmuth,K., Urlaub,H., Kastner,B., Stark,H. and Luhrmann,R.
TITLE Cryo-EM structure of a human spliceosome activated for step 2 of
splicing
JOURNAL Nature 542 (7641), 318-323 (2017)
PUBMED 28076346
REFERENCE 4 (bases 1 to 4406)
AUTHORS Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
Landthaler,M.
TITLE The mRNA-bound proteome and its global occupancy profile on
protein-coding transcripts
JOURNAL Mol Cell 46 (5), 674-690 (2012)
PUBMED 22681889
REFERENCE 5 (bases 1 to 4406)
AUTHORS Hegele,A., Kamburov,A., Grossmann,A., Sourlis,C., Wowro,S.,
Weimann,M., Will,C.L., Pena,V., Luhrmann,R. and Stelzl,U.
TITLE Dynamic protein-protein interaction wiring of the human spliceosome
JOURNAL Mol Cell 45 (4), 567-580 (2012)
PUBMED 22365833
REFERENCE 6 (bases 1 to 4406)
AUTHORS Hillman,R.T., Green,R.E. and Brenner,S.E.
TITLE An unappreciated role for RNA surveillance
JOURNAL Genome Biol 5 (2), R8 (2004)
PUBMED 14759258
REFERENCE 7 (bases 1 to 4406)
AUTHORS Chung,S., Zhou,Z., Huddleston,K.A., Harrison,D.A., Reed,R.,
Coleman,T.A. and Rymond,B.C.
TITLE Crooked neck is a component of the human spliceosome and implicated
in the splicing process
JOURNAL Biochim Biophys Acta 1576 (3), 287-297 (2002)
PUBMED 12084575
REMARK GeneRIF: Data show that crooked neck activity appears essential as
extracts depleted of hcrn fail to splice pre-mRNA. These and
related data support the view that crooked neck is a
phylogenetically conserved pre-mRNA splicing factor.
REFERENCE 8 (bases 1 to 4406)
AUTHORS Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
Moore,M.J.
TITLE Purification and characterization of native spliceosomes suitable
for three-dimensional structural analysis
JOURNAL RNA 8 (4), 426-439 (2002)
PUBMED 11991638
REFERENCE 9 (bases 1 to 4406)
AUTHORS Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
Rogers,J.
TITLE The DNA sequence and comparative analysis of human chromosome 20
JOURNAL Nature 414 (6866), 865-871 (2001)
PUBMED 11780052
REFERENCE 10 (bases 1 to 4406)
AUTHORS Lai,C.H., Chiu,J.Y. and Lin,W.
TITLE Identification of the human crooked neck gene by comparative gene
identification
JOURNAL Biochim Biophys Acta 1517 (3), 449-454 (2001)
PUBMED 11342225
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AF318303.1, AL035454.18,
BC144222.1, AL832435.1 and AA255987.1.
On Aug 13, 2020 this sequence version replaced NM_016652.5.
Summary: The crooked neck (crn) gene of Drosophila is essential for
embryogenesis and is thought to be involved in cell cycle
progression and pre-mRNA splicing. A protein encoded by this human
locus has been found to localize to pre-mRNA splicing complexes in
the nucleus and is necessary for pre-mRNA splicing. Alternatively
spliced transcript variants have been described. [provided by
RefSeq, Jul 2013].
Transcript Variant: This variant (1) represents the longest
transcript and encodes the longest isoform (a).
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AF318303.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA1965299,
SAMEA1966682 [ECO:0006172]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-121 AF318303.1 1-121
122-135 AL035454.18 34048-34061 c
136-614 AF318303.1 136-614
615-671 BC144222.1 575-631
672-3288 AF318303.1 672-3288
3289-4225 AL832435.1 2898-3834
4226-4406 AA255987.1 13-193 c
FEATURES Location/Qualifiers
source 1..4406
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="20"
/map="20p11.23"
gene 1..4406
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="crooked neck pre-mRNA splicing factor 1"
/db_xref="GeneID:51340"
/db_xref="HGNC:HGNC:15762"
/db_xref="MIM:610952"
exon 1..99
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
CDS 33..2579
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="isoform a is encoded by transcript variant 1;
crooked neck-like 1; crooked neck-like protein 1; crooked
neck homolog; Crn, crooked neck-like 1; SYF3
pre-mRNA-splicing factor; crooked neck pre-mRNA splicing
factor-like 1"
/codon_start=1
/product="crooked neck-like protein 1 isoform a"
/protein_id="NP_057736.4"
/db_xref="CCDS:CCDS33446.1"
/db_xref="GeneID:51340"
/db_xref="HGNC:HGNC:15762"
/db_xref="MIM:610952"
/translation="MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLV
LAWSSQFRRLTWGCALDALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHG
NGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAA
DMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEE
ELNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNI
TLWLKYAEMEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFE
RWMEWQPEEQAWHSYINFELRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYF
AHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQDAQE
LFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEAE
AVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIP
HKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDR
CRKLYEKFLEFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSY
IDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANK
TMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKVKKRRKVQTDDGSDAGW
EEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES"
misc_feature 36..38
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="N-acetylalanine.
/evidence=ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (Q9BZJ0.4); acetylation site"
misc_feature 273..350
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 696..794
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 1"
misc_feature 798..896
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 2"
misc_feature 900..998
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 3"
misc_feature 1002..1097
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 4"
misc_feature 1101..1196
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 5"
misc_feature 1200..1307
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 6"
misc_feature 1263..1916
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: Mediates interaction with HSP90.
/evidence=ECO:0000250|UniProtKB:P63154"
misc_feature 1539..1541
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (Q9BZJ0.4); phosphorylation site"
misc_feature 1890..1988
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 12"
misc_feature 1992..2096
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 13"
misc_feature 2100..2195
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 14"
misc_feature 2208..2333
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 15"
misc_feature 2337..2453
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 16"
misc_feature 2457..2534
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: HAT 17"
misc_feature 2511..2576
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="propagated from UniProtKB/Swiss-Prot (Q9BZJ0.4);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
exon 100..566
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 567..719
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 720..811
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 812..970
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 971..1137
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 1138..1316
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 1317..1487
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 1488..1679
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 1680..1739
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 1740..1820
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 1821..2060
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 2061..2162
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 2163..2411
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
exon 2412..4406
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/inference="alignment:Splign:2.1.0"
regulatory 2613..2618
/regulatory_class="polyA_signal_sequence"
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="hexamer: TATAAA"
polyA_site 2632
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="major polyA site"
regulatory 3408..3413
/regulatory_class="polyA_signal_sequence"
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="hexamer: CATAAA"
polyA_site 3429
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
regulatory 3967..3972
/regulatory_class="polyA_signal_sequence"
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="hexamer: AATAAA"
polyA_site 3997
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
regulatory 4378..4383
/regulatory_class="polyA_signal_sequence"
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
/note="hexamer: AGTAAA"
polyA_site 4406
/gene="CRNKL1"
/gene_synonym="CLF; Clf1; CRN; HCRN; MSTP021; SYF3"
ORIGIN
1 ctttccgaca ctctttggga agagaatctc taatgacggc aacagtggag aacctaacgt
61 tccagaagga cactttggga aatgctgttg acaaaaacac atccagattg gagctccgtt
121 cttactccct tgcagggcgc cacggctcga cagagccact cgtcctcgcc tggtcatccc
181 agttccggag gctgacctgg ggatgcgccc tggacgccct gcaccgcagt ccttgcgtcg
241 ctgctagtca gcacggcgtt acccacctca tccgcagctc caggacgccg cactccacgc
301 gctgccgcaa ggaggacgcg cagcctggtc accatggaaa cggtgccgcc tccgtcacgg
361 ctcaagcgag aggtcaaagg tcagtcctgc aagttccgct tccggttccg agatcctgtt
421 tgttttctga aagctttgtg gtttcggtga gctctcagag ccgatttcta gcgtccgtgc
481 cggggacagg tgtccagagg tcgactgctg cagacatggc ggcctccacc gcggccggga
541 agcagcggat tcccaaagtg gccaaggtga aaaacaaagc cccggctgag gtacagataa
601 ctgctgaaca actcttaaga gaggctaaag aaagagaact tgagcttctt ccacctccac
661 ctcaacagaa gatcacagat gaagaagaat taaatgatta taaactaagg aaaaggaaga
721 cttttgaaga taatataaga aaaaacagga ctgtgattag taactggata aaatacgcac
781 aatgggaaga aagcctaaag gagattcaaa gggctcgatc catatacgag cgtgctttag
841 atgtagacta ccgaaatatt acactctggc tgaaatacgc agaaatggaa atgaagaatc
901 gccaagtcaa ccatgctcga aatatctggg accgggccat aacaacgctg cctcgagtta
961 atcagttctg gtacaagtac acgtacatgg aggaaatgtt gggaaacgtt gccggtgccc
1021 ggcaggtgtt tgagcgctgg atggagtggc agcctgagga gcaagcctgg cactcctaca
1081 tcaactttga gctgagatac aaagaggtgg atcgggcccg caccatttat gagcgatttg
1141 tcctcgtgca ccctgatgtt aagaactgga tcaagtatgc ccgctttgaa gaaaaacatg
1201 cttattttgc ccatgcacgg aaagtgtatg agagagctgt ggaattcttt ggagatgaac
1261 atatggatga gcacctttat gttgcctttg ccaagtttga agaaaatcag aaagagtttg
1321 aaagggtacg agtgatttac aagtatgccc tggacagaat ttcaaaacaa gatgcccaag
1381 aactctttaa aaattatacc atctttgaga agaagtttgg tgataggcgg ggtattgaag
1441 atatcattgt gagcaaacgg agattccagt acgaagaaga agtgaaggcg aatccacaca
1501 attatgatgc atggtttgat tacttgcgct tggtagaaag tgacgcagaa gctgaagccg
1561 tgagagaagt ctatgaaagg gccattgcca atgtcccacc cattcaggag aagaggcact
1621 ggaagcgcta catttatctt tggatcaact atgcactcta tgaagaattg gaggcaaagg
1681 atcctgagag gacaagacag gtgtatcaag cctctttgga actaattcct cacaaaaagt
1741 tcacatttgc caaaatgtgg atactgtatg cacagtttga aatacgacag aagaatctgt
1801 cattagccag aagagcattg ggaacttcca taggcaaatg tccaaagaac aaattattta
1861 aagtttacat agaattggag ctacagcttc gagaatttga cagatgccgg aagctttatg
1921 aaaagttcct ggaatttgga cctgaaaatt gtacctcatg gattaaattc gctgaattag
1981 agacaatcct tggtgatatt gacagagcac gggcaatcta tgaattagcc atcagtcagc
2041 cacgtttaga catgccagag gtgctttgga aatcatatat tgattttgaa attgagcagg
2101 aagaaacaga aagaacacga aacctttacc ggcggttgct tcaacggacg cagcatgtca
2161 aggtatggat cagctttgct cagtttgagt tgtcttcagg aaaagaagga agtttgacta
2221 aatgcagaca aatttatgaa gaagctaaca aaaccatgcg aaactgtgaa gaaaaggaag
2281 agagacttat gctgctggaa tcttggcgaa gttttgaaga agaatttgga acagcttcag
2341 ataaggagag agtagacaaa ctcatgccag agaaagtcaa gaagagaaga aaggtccaga
2401 ctgatgatgg gtctgatgca ggctgggaag aatactttga ttacatcttt ccagaagatg
2461 ctgccaacca acctaacctc aaactcctgg ccatggccaa actgtggaag aaacagcagc
2521 aggaaaagga ggatgctgag caccatccag atgaggacgt cgatgagagt gaatcctgat
2581 ctttttttca tagacaaatg ttttgttatt tttataaatt aattgtttgg aactcttgtg
2641 actcctggaa gttcttatat atttcaccag taagaaattg attggtatct ttgatggcta
2701 ctttttaagt tattttttaa atgctcttgg gttagctagg ggtagggatt gcaagtaaag
2761 gactttttta actgctggat ttgtttttcc aactgagtcc aaacttttct aatgtctgtc
2821 cacatcatgc attaggaaat gtaattaagg taacattcta cagttacttt tcatgtcata
2881 cccataaaga tagtttatgc attcatctga aatgtgtaac tttttcatgt cttcagagtc
2941 acagacttga gttcatttcc cagctctgcc actcatgatt atataactta attttcattt
3001 tcctcattca caaaatgggc caatagtttg acagctcatt ttgaagatta cattataaaa
3061 ggaatatacc tggtgggtgc atagtaagtg ctcagtaaat tgtttgttct aagccacttt
3121 taaaaatggt ttcattcctt gtagaattga atgcgagtgg attaataatt taccttactt
3181 tcttactagt gtccagttat attgtttttt agaacaacac ttggaaaata atttgcagtg
3241 attatatttc tgaacaaggt tcagaaaaca ttgtttacta agaatttagt ctaataattt
3301 cagttaggcg ctcatcagtt ctccagagtg gttgagtttg taataccttg tttaaagaat
3361 aatggcttgt tcacgtgtgt gctatgaaaa atgatgtccc atgttcacat aaatttggga
3421 aattctggac taagacttaa gtctcgttaa tcaaatctct ttatagttag gcttctgtac
3481 attatgtatc tccagtagca atgttgccat attatttatt tcccaaactt agtggacaat
3541 ggagtcattt ctacctagag taccagtaaa catctcccag tgtgctatag tagaaaatgt
3601 ctactcctca ctgctgacat gttaaactta ctcttggttt agagcatgtg tagaaacacc
3661 taaggtagct ctatgctaaa taatgaagag tagcacaaga atgaatgtat ttgctgatac
3721 gttgctcaca ttctcaagca aaaattcaac tgcattaacc gatctgagag ttttccttta
3781 acctggactg tgtttctcaa gcacattttt tctttgttca ctgcccaagg actagaactg
3841 tatttttaag gttgttttcc cctaaaagga cctttagtaa gcaaatttat tattaaatgt
3901 gcacatctta ttcacccaag ggaataaaag ctacttcgta atgttgttac taaattttat
3961 cttgaaaata aataacagtg tttgaggaca gaagaaacta ggactgttgt tgagataaaa
4021 tctgttttca gcctgactgc tttagtcact ggacgagcat cagctactgc ggtgtcagat
4081 cacttctcga gtagaggtct gcctagtgag tgagcaggag aacatcatct taatagcctt
4141 tggctcaccc agagtcaggg agagatgaaa ataataggtc agaatgcaag tgacttaatc
4201 acagtcttcc tagaattatc tgtttatgtt gcatgaatgc ataagaaaaa aattctgtca
4261 tgttcccaag gtagaattgt aataagcatg tgccagtgtg aaagctggaa ggaaaaaagc
4321 tattctctaa caggagaaca taaagattca tttctctccc accctcaact ccaccaaagt
4381 aaatcctttc gatagtaaaa gtttaa
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