LOCUS BQ632184 390 bp mRNA linear EST 02-JUL-2002
DEFINITION il23f02.x1 HR85 islet Homo sapiens cDNA clone IMAGE:6030866 3',
mRNA sequence.
ACCESSION BQ632184
VERSION BQ632184.1
DBLINK BioSample: SAMN00163360
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 390)
AUTHORS Melton,D., Brown,J., Kenty,G., Permutt,A., Lee,C., Kaestner,K.,
Lemishka,I., Scearce,M., Brestelli,J., Gradwohl,G., Clifton,S.,
Hillier,L., Marra,M., Pape,D., Wylie,T., Martin,J., Blistain,A.,
Schmitt,A., Theising,B., Ritter,E., Ronko,I., Bennett,J.,
Cardenas,M., Gibbons,M., McCann,R., Cole,R., Tsagareishvili,R.,
Williams,T., Jackson,Y. and Bowers,Y.
TITLE Endocrine Pancreas Consortium
JOURNAL Unpublished
COMMENT Other_ESTs: il23f02.y1
Contact: Douglas Melton, Klaus H. Kaestner, & Hiroshi Inoue
Endocrine Pancreas Consortium
Harvard University, Howard Hughes Medical Institute
Dept of Molecular and Cellular Biology, 7 Divinity Ave, Cambridge,
MA 02138
Tel: 617-495-1812
Fax: 617-495-8557
Email: dmelton@biohp.harvard.edu
Library was constructed by Dr. Hiroshi Inoue DNA sequencing by:
Washington University Genome Sequencing Center For information on
obtaining a clone please contact: Dr. Hiroshi Inoue
(hinoue@im.wustl.edu)
Seq primer: -40UP from Gibco
High quality sequence stop: 377.
FEATURES Location/Qualifiers
source 1..390
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6030866"
/tissue_type="Purified pancreatic islet"
/clone_lib="SAMN00163360 HR85 islet"
/lab_host="DH10B"
/note="Organ: Pancreas; Vector: pBluescript SK(-); Site_1:
NotI; Site_2: XhoI; cDNA made by oligo-dT priming.
Size-selected on agarose gel. Average insert size
1kb. 5' XhoI site was destroyed after directional cloning.
Amplified once. Contact information: Hiroshi Inoue, MD,
Metabolism Div. (Alan Permutt Lab), Washington University
School of Medicine, Box 8127, 660 South Euclid Ave., St.
Louis, MO 63110, E-mail: hinoue@imgate.wustl.edu, Tel:
314-362-1916, Fax: 314-747-2692."
ORIGIN
1 tttttttttt ttttcaacta ccgtgattgc atttattctt ataaatgtac agagctgtag
61 aagtgcaagc caagagttct atagagtagt acataaacac catatggtac cactcctgct
121 gggaggtaag cctggataca cccctctcct caggaaactg tcacctgcag aacacacagc
181 actcaaaatt aaggcagttt ggccctgggc acattggttg ttttttggta tgtggccact
241 ggccctaaac aactgaccat ttctaccctg cctcactgca ctgtcccacc aggtccctcc
301 agctttttct acaaggtaac accctcctac atggggtcag ccagctcaga aacctcttct
361 tcagggacag ttgcagctga atatgccaga
//