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GenBank: CB322460.1
FASTA Graphics
LOCUS CB322460 774 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-DY0-crb-p-13-0-UI.s1 NCI_CGAP_DY0 Rattus norvegicus cDNA clone IMAGE:7329759 3', mRNA sequence. ACCESSION CB322460 VERSION CB322460.1 DBLINK BioSample: SAMN00170067 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 774) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Daniela S. Gerhard, Ph.D. Office of Cancer Genomics National Cancer Institute / NIH Bldg. 31 Rm10A07 Bethesda, MD 20892 Email: cgapbs-r@mail.nih.gov Oligo-dT track not found, Not I site shown in beginning of sequence is likely internal to the message. cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through IMAGE (http://image.llnl.gov) Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..774 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="IMAGE:7329759" /clone_lib="SAMN00170067 NCI_CGAP_DY0" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; UI-R-DY0 is a non-normalized Rat cartilage library (RC) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CTAATGGACG between the Not I cloning site and dT18 stretch. The Rat cartilage tissue was provided by Dr Jeff Stevens at the University of Iowa. TAG_LIB=UI-R-DY0; TAG_SEQ=CTAATGGACG; TAG_TISSUE=cartilage" ORIGIN 1 tttttttttt ttttttttcc agtggagagc acaaatgtat tttggaatct gcttgttaac 61 ttgggtagca agccagcaga ggtcccatag ccagctcgcc aaccacccag agcccatcta 121 tctctccaga cccgacagtc ctcttgtcaa ccgaggctcc agagcgaggg tcaagagggg 181 tctcaggaga taagaggcag gatctggtga cccaaggggc agcaagcgga cgtgggcctc 241 tccttccagg aatggaacat ggagggatgt gcggtgtgca gagacgggtg ggcaaccaga 301 ggttcccttt agtcttgagt caacccctgg agaaagttta ggacttgggg tggttggtac 361 agcagcccac ataccccaac ttcactcttc ttctctattc atctcagacc tgtccaactc 421 ccccagaatg cctgagagaa ggggctagca ggaggcctgt gggggctgca gggtgagggg 481 aagggtgagg ggcaactctg aaaccccctt gcccttcctg cctgtagtcc cagtgcaatc 541 aagagggagg attgagaagg tgtcagccac agaaggcagc agggcctggg aggcagggcc 601 aggaggcccc tcagcctctg gccgtcctcg tcacttgggc tggaaggact gggcactaac 661 agcccagcag ctccaggcgc agggtgatac ggttatgcca ggacagtgga aggacacgca 721 catagcgagc catgaaaggt ttctcanaga tgttcttctt gtngggagtt gttt //
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