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UI-R-DY0-crb-p-13-0-UI.s1 NCI_CGAP_DY0 Rattus norvegicus cDNA clone IMAGE:7329759 3', mRNA sequence

GenBank: CB322460.1

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LOCUS       CB322460                 774 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-DY0-crb-p-13-0-UI.s1 NCI_CGAP_DY0 Rattus norvegicus cDNA clone
            IMAGE:7329759 3', mRNA sequence.
ACCESSION   CB322460
VERSION     CB322460.1
DBLINK      BioSample: SAMN00170067
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Daniela S. Gerhard, Ph.D.
            Office of Cancer Genomics
            National Cancer Institute / NIH
            Bldg. 31 Rm10A07 Bethesda, MD 20892
            Email: cgapbs-r@mail.nih.gov
            Oligo-dT track not found, Not I site shown in beginning of sequence
            is likely internal to the message. cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            IMAGE (http://image.llnl.gov)
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="IMAGE:7329759"
                     /clone_lib="SAMN00170067 NCI_CGAP_DY0"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     UI-R-DY0 is a non-normalized Rat cartilage library (RC)
                     constructed in pT3T7 PAC vector according to the procedure
                     described by Bonaldo, Lennon & Soares (Genome Research
                     Genome 6: 791-806, 1996). The oligonucleotide used to
                     prime first strand synthesis contained the sequence tag
                     CTAATGGACG between the Not I cloning site and dT18
                     stretch. The Rat cartilage tissue was provided by Dr Jeff
                     Stevens at the University of Iowa.
                     TAG_LIB=UI-R-DY0;
                     TAG_SEQ=CTAATGGACG;
                     TAG_TISSUE=cartilage"
ORIGIN      
        1 tttttttttt ttttttttcc agtggagagc acaaatgtat tttggaatct gcttgttaac
       61 ttgggtagca agccagcaga ggtcccatag ccagctcgcc aaccacccag agcccatcta
      121 tctctccaga cccgacagtc ctcttgtcaa ccgaggctcc agagcgaggg tcaagagggg
      181 tctcaggaga taagaggcag gatctggtga cccaaggggc agcaagcgga cgtgggcctc
      241 tccttccagg aatggaacat ggagggatgt gcggtgtgca gagacgggtg ggcaaccaga
      301 ggttcccttt agtcttgagt caacccctgg agaaagttta ggacttgggg tggttggtac
      361 agcagcccac ataccccaac ttcactcttc ttctctattc atctcagacc tgtccaactc
      421 ccccagaatg cctgagagaa ggggctagca ggaggcctgt gggggctgca gggtgagggg
      481 aagggtgagg ggcaactctg aaaccccctt gcccttcctg cctgtagtcc cagtgcaatc
      541 aagagggagg attgagaagg tgtcagccac agaaggcagc agggcctggg aggcagggcc
      601 aggaggcccc tcagcctctg gccgtcctcg tcacttgggc tggaaggact gggcactaac
      661 agcccagcag ctccaggcgc agggtgatac ggttatgcca ggacagtgga aggacacgca
      721 catagcgagc catgaaaggt ttctcanaga tgttcttctt gtngggagtt gttt
//
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