LOCUS NM_002859 3691 bp mRNA linear PRI 01-MAY-2019
DEFINITION Homo sapiens paxillin (PXN), transcript variant 1, mRNA.
ACCESSION NM_002859 XM_001132665 XM_001134353
VERSION NM_002859.3
KEYWORDS RefSeq; RefSeq Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3691)
AUTHORS Bozic J, Stoka V and Dolenc I.
TITLE Glucosamine prevents polarization of cytotoxic granules in NK-92
cells by disturbing FOXO1/ERK/paxillin phosphorylation
JOURNAL PLoS ONE 13 (7), e0200757 (2018)
PUBMED 30016365
REMARK GeneRIF: Glucosamine affects signaling pathway activation of NK-92
immune cells, changing the phosphorylation of FOXO1, paxillin, and
the ERK map kinase pathway
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3691)
AUTHORS Zhang LL, Zhang LF and Shi YB.
TITLE miR-24 inhibited the killing effect of natural killer cells to
colorectal cancer cells by downregulating Paxillin
JOURNAL Biomed. Pharmacother. 101, 257-263 (2018)
PUBMED 29494963
REMARK GeneRIF: overexpression of Paxillin significantly decreased tumor
volume of colorectal cancer; miR-24 was overexpressed in natural
killer cells and inhibited paxillin expression.
REFERENCE 3 (bases 1 to 3691)
AUTHORS Jiang H, Zhang H, Hu X and Li W.
TITLE Knockdown of long non-coding RNA XIST inhibits cell viability and
invasion by regulating miR-137/PXN axis in non-small cell lung
cancer
JOURNAL Int. J. Biol. Macromol. 111, 623-631 (2018)
PUBMED 29337100
REMARK GeneRIF: XIST positively regulated PXN levels by sponging miR-137
in vitro and in vivo. Collectively, our study provided the evidence
for the cross-talk between XIST, miR-137, and PXN, shedding light
on the therapy for non-small cell lung cancer .
REFERENCE 4 (bases 1 to 3691)
AUTHORS Liu Q, Wang J, Tang M, Chen L, Qi X, Li J, Yu J, Qiu H and Wang Y.
TITLE The overexpression of PXN promotes tumor progression and leads to
radioresistance in cervical cancer
JOURNAL Future Oncol 14 (3), 241-253 (2018)
PUBMED 29318915
REMARK GeneRIF: we herein demonstrated that frequent overexpression of PXN
in cervical cancer was associated with advanced tumor stage, poor
differentiation and metastasis, and poor predictive outcomes
REFERENCE 5 (bases 1 to 3691)
AUTHORS Montone R, Romanelli MG, Baruzzi A, Ferrarini F, Liboi E and
Lievens PM.
TITLE Mutant FGFR3 associated with SADDAN disease causes cytoskeleton
disorganization through PLCgamma1/Src-mediated paxillin
hyperphosphorylation
JOURNAL Int. J. Biochem. Cell Biol. 95, 17-26 (2018)
PUBMED 29242050
REMARK GeneRIF: Data suggest that FGFR3 with mutation found in patients
with SADDAN (but not FGFR3 with mutation found in patients with
TDII) affects cytoskeleton organization in chondrocytes by inducing
tyrosine hyperphosphorylation of paxillin. (FGFR3 = fibroblast
growth factor receptor 3; SADDAN = Severe Achondroplasia with
Developmental Delay and Acanthosis Nigricans; TDII = Thanatophoric
Dysplasia type II)
REFERENCE 6 (bases 1 to 3691)
AUTHORS Ganju RK, Munshi N, Nair BC, Liu ZY, Gill P and Groopman JE.
TITLE Human immunodeficiency virus tat modulates the Flk-1/KDR receptor,
mitogen-activated protein kinases, and components of focal adhesion
in Kaposi's sarcoma cells
JOURNAL J. Virol. 72 (7), 6131-6137 (1998)
PUBMED 9621077
REFERENCE 7 (bases 1 to 3691)
AUTHORS Tong X and Howley PM.
TITLE The bovine papillomavirus E6 oncoprotein interacts with paxillin
and disrupts the actin cytoskeleton
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 94 (9), 4412-4417 (1997)
PUBMED 9114003
REFERENCE 8 (bases 1 to 3691)
AUTHORS Mazaki Y, Hashimoto S and Sabe H.
TITLE Monocyte cells and cancer cells express novel paxillin isoforms
with different binding properties to focal adhesion proteins
JOURNAL J. Biol. Chem. 272 (11), 7437-7444 (1997)
PUBMED 9054445
REFERENCE 9 (bases 1 to 3691)
AUTHORS Tachibana K, Sato T, D'Avirro N and Morimoto C.
TITLE Direct association of pp125FAK with paxillin, the focal
adhesion-targeting mechanism of pp125FAK
JOURNAL J. Exp. Med. 182 (4), 1089-1099 (1995)
PUBMED 7561682
REFERENCE 10 (bases 1 to 3691)
AUTHORS Salgia R, Li JL, Lo SH, Brunkhorst B, Kansas GS, Sobhany ES, Sun Y,
Pisick E, Hallek M, Ernst T et al.
TITLE Molecular cloning of human paxillin, a focal adhesion protein
phosphorylated by P210BCR/ABL
JOURNAL J. Biol. Chem. 270 (10), 5039-5047 (1995)
PUBMED 7534286
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from DB472926.1, BC136794.1,
BU500419.1, BC144410.1, BX648777.1 and AA922640.1.
[WARNING] On Jun 2, 2019 this sequence was replaced by NM_002859.4.
On Aug 25, 2011 this sequence version replaced NM_002859.2.
Summary: This gene encodes a cytoskeletal protein involved in
actin-membrane attachment at sites of cell adhesion to the
extracellular matrix (focal adhesion). Alternatively spliced
transcript variants encoding different isoforms have been described
for this gene. These isoforms exhibit different expression pattern,
and have different biochemical, as well as physiological properties
(PMID:9054445). [provided by RefSeq, Aug 2011].
Transcript Variant: This variant (1) represents the predominant
transcript, and encodes isoform 1 (also known as isoform alpha).
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: U14588.1, SRR1803615.120200.1
[ECO:0000332]
RNAseq introns :: mixed/partial sample support
SAMEA1965299, SAMEA1966682
[ECO:0000350]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on conservation, expression
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-66 DB472926.1 3-68
67-358 BC136794.1 1-292
359-359 BU500419.1 292-292
360-1962 BC136794.1 294-1896
1963-2653 BC144410.1 1999-2689
2654-3683 BX648777.1 2771-3800
3684-3691 AA922640.1 1-8 c
FEATURES Location/Qualifiers
source 1..3691
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="12"
/map="12q24.23"
gene 1..3691
/gene="PXN"
/note="paxillin"
/db_xref="GeneID:5829"
/db_xref="HGNC:HGNC:9718"
/db_xref="MIM:602505"
exon 1..155
/gene="PXN"
/inference="alignment:Splign:2.1.0"
misc_feature 119..121
/gene="PXN"
/note="upstream in-frame stop codon"
CDS 143..1816
/gene="PXN"
/note="isoform 1 is encoded by transcript variant 1;
testicular tissue protein Li 134"
/codon_start=1
/product="paxillin isoform 1"
/protein_id="NP_002850.2"
/db_xref="CCDS:CCDS44996.1"
/db_xref="GeneID:5829"
/db_xref="HGNC:HGNC:9718"
/db_xref="MIM:602505"
/translation="MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIA
VPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQD
SVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPP
GFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVE
SLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDF
KFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQ
VVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILD
KVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAIL
ENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPI
TGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC"
exon 156..382
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 383..498
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 499..635
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 636..837
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 838..973
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 974..1144
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 1145..1318
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 1319..1400
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 1401..1549
/gene="PXN"
/inference="alignment:Splign:2.1.0"
exon 1550..3691
/gene="PXN"
/inference="alignment:Splign:2.1.0"
regulatory 3660..3665
/regulatory_class="polyA_signal_sequence"
/gene="PXN"
polyA_site 3683
/gene="PXN"
ORIGIN
1 ggcgggcgcg ggcgcgggcg cgcgcggcgg cggcgcgttc cgttccgggc cgaggctcgc
61 ggcggaaaag ttgcggggca tagacgagcg gccccgggac gggcagctag cgcgaccctg
121 agccggcgcc cgtggtccgg ccatggacga cctcgacgcc ctgctggcgg acttggagtc
181 taccacctcc cacatctcca aacggcctgt gttcttgtcg gaggagaccc cctactcata
241 cccaactgga aaccacacat accaggagat tgccgtgcca ccccccgtcc ccccaccccc
301 gtccagcgag gccctcaatg gcacaatcct tgacccctta gaccagtggc agcccagcag
361 ctcccgattc atccaccagc agcctcagtc ctcatcacct gtgtacggct ccagtgccaa
421 aacttccagt gtctccaacc ctcaggacag tgttggctct ccgtgctccc gagtgggtga
481 ggaggagcac gtctacagct tccccaacaa gcagaaatca gctgagcctt cacccaccgt
541 aatgagcacg tccctgggca gcaacctttc tgaactcgac cgcctgctgc tggaactgaa
601 cgctgtacag cataacccgc caggcttccc tgcagatgag gccaactcaa gccccccgct
661 tcctggggcc ctgagccccc tctatggtgt cccagagact aacagcccct tgggaggcaa
721 agctgggccc ctgacgaaag agaagcctaa gcggaatggg ggccggggcc tggaggacgt
781 gcggcccagt gtggagagtc tcttggatga actggagagc tccgtgccca gccccgtccc
841 tgccatcact gtgaaccagg gcgagatgag cagcccgcag cgcgtcacct ccacccaaca
901 gcagacacgc atctcggcct cctctgccac cagggagctg gacgagctga tggcttcgct
961 gtcggatttc aagttcatgg cccaggggaa gacagggagc agctcacccc ctggggggcc
1021 cccgaagccc gggagccagc tggacagcat gctggggagc ctgcagtctg acctgaacaa
1081 gctgggggtc gccacagtcg ccaaaggagt ctgcggggcc tgcaagaagc ccatcgccgg
1141 gcaggttgtg accgccatgg ggaagacgtg gcaccccgag cacttcgtct gcacccactg
1201 ccaggaggag atcggatccc ggaacttctt cgagcgggat ggacagccct actgtgaaaa
1261 ggactaccac aacctcttct ccccgcgctg ctactactgc aacggcccca tcctggataa
1321 agtggtgaca gcccttgacc ggacgtggca ccctgaacac ttcttctgtg cacagtgtgg
1381 agccttcttt ggtcccgaag ggttccacga gaaggacggc aaggcctact gtcgcaagga
1441 ctacttcgac atgttcgcac ccaagtgtgg cggctgcgcc cgggccatcc tggagaacta
1501 tatctcagcc ctcaacacgc tgtggcatcc tgagtgcttt gtgtgccggg aatgcttcac
1561 gccattcgtg aacggcagct tcttcgagca cgacgggcag ccctactgtg aggtgcacta
1621 ccacgagcgg cgcggctcgc tgtgttctgg ctgccagaag cccatcaccg gccgctgcat
1681 caccgccatg gccaagaagt tccaccccga gcacttcgtc tgtgccttct gcctcaagca
1741 gctcaacaag ggcaccttca aggagcagaa cgacaagcct tactgtcaga actgcttcct
1801 caagctcttc tgctaggtgc cctgcccctg tctctgcccc ccttccccag ccagcatccc
1861 caactgcgac tgtgacctag agacttcacc cgggggtgaa ggggtaaacc cgactgaaac
1921 tggaaccctt gtcctccgct ggtgcgggat ggacagaggg ccgtgagggg tccccctgct
1981 tgtcttcacc cctgccagag cctctgggcc ccctcctccc tcctgtagct ctccctaggc
2041 tgcccactct ccatcctccc caggggtaga ggctgggggc tccaccccag cccatgtacg
2101 tccccacgaa ctggcctggc cagcacccca cactggagcc atctcttcct catatttcag
2161 cagtgcagcc ggggggcagg gaagggcagg cagggtctgt tggggtctct ttttatcctt
2221 attcctcccc cgacctaatt gtctttgttc tgtgattatt gggggacacc cggctccctc
2281 cagacaatgc cagcataaat ccatccatcc aaaggcagag aaccaaaggg gccatggaag
2341 gttctctgtg ctcctcctac ccttccagtg ccctaggcct ggcgactgcc cctgcctttt
2401 agacccgccc tcccctttta tacctgctct tgttctactg agaaaagcct ctccagcaat
2461 aatgttttct agtcacttcc tccgtctccg ggacggcgtg cctggacact gtaccgactt
2521 tgatagattt ctacactgag gtttgaattc atatcgcctg agttgctttt acttctctat
2581 acaaaatgat tttgaagaga ttttaaagac gttccctttt gtattctctt cctcatccac
2641 cgccactggg cctgtcactg atggtggctc tggtgtgaag tttgctttgt actgagggtt
2701 ggggtgggga agcaatttgt attttattgt ttcttagcac aagcaggtga actgggagca
2761 gctctgtgac tccccctctt tcacttcata gctcaccagg actgttttat aaactgctgt
2821 atttggaaac cccttcttta cttcccaggc cagcaagctc ttcactgaaa ctggttgaag
2881 ggtgttgcac ccttttgggc ctagaattct gaactttatc tgttctgttt ctgtgggagg
2941 agaaggggaa gtatgttttg gggggctgct tcctgtctga gtaagccctc aggagcctct
3001 gctcccctgt gaacccactg aaccttctga gcccccactg cttctatggg gctctctctt
3061 ctgccttctc aggaaagctg gtgtctgatt ttggccatca ggactctgac gtctctttgg
3121 tcttgttgat ttcctctggg catatccctt ccccagatct gctcctcccc tttcacaggt
3181 gggatcggca ctcagggggt ctggaaagaa ggtcataagg gagcatgata ggatttgggg
3241 cagagggaca ggctcctctg gggaaacccc ccagagctct ttaccaagga tgaaagagga
3301 gccaggcctt gggctcctga tgaggagaaa ggggccccac cggggtctaa tggtgacagt
3361 ccaaaccact ccactggcct cctggcagaa gccgagtgtg ctggggtctc cgaagagggt
3421 ccctcctttt tgggggaagg tcagcccagc ccctccaaag gtctgatgtc tccactttca
3481 cccgcaggcc ttaccgctct gtttatagtg acccacccta gatcttcccc aagagggact
3541 ggggtttctg gggtccattc tctgagtcag tggttatttg aaaatttgat tttgatttta
3601 ttttttctct gtaaacttcc aagctggctt ttcccatttc aattcctgtg atttatgcca
3661 ataaagtttg cccatgattt tcacctgtgc t
//