LOCUS NM_001183755 2877 bp mRNA linear PLN 17-DEC-2024
DEFINITION Saccharomyces cerevisiae S288C alanine--tRNA ligase (ALA1), partial
mRNA.
ACCESSION NM_001183755
VERSION NM_001183755.3
DBLINK BioProject: PRJNA128
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 2877)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 2877)
AUTHORS Dujon,B., Albermann,K., Aldea,M., Alexandraki,D., Ansorge,W.,
Arino,J., Benes,V., Bohn,C., Bolotin-Fukuhara,M., Bordonne,R.,
Boyer,J., Camasses,A., Casamayor,A., Casas,C., Cheret,G.,
Cziepluch,C., Daignan-Fornier,B., Dang,D.V., de Haan,M., Delius,H.,
Durand,P., Fairhead,C., Feldmann,H., Gaillon,L., Kleine,K. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome XV
JOURNAL Nature 387 (6632 SUPPL), 98-102 (1997)
PUBMED 9169874
REFERENCE 3 (bases 1 to 2877)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (bases 1 to 2877)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (17-DEC-2024) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 2877)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2015) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 2877)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 7 (bases 1 to 2877)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 8 (bases 1 to 2877)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (27-MAY-2010) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 9 (bases 1 to 2877)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. This record
is derived from an annotated genomic sequence (NC_001147).
On Jul 30, 2012 this sequence version replaced NM_001183755.2.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: incomplete on both ends.
FEATURES Location/Qualifiers
source 1..2877
/organism="Saccharomyces cerevisiae S288C"
/mol_type="mRNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="XV"
gene <1..>2877
/gene="ALA1"
/locus_tag="YOR335C"
/gene_synonym="CDC64"
/db_xref="GeneID:854513"
CDS 1..2877
/gene="ALA1"
/locus_tag="YOR335C"
/gene_synonym="CDC64"
/EC_number="6.1.1.7"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16556230|PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:24769239|PMID:16556230|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:mutant phenotype:GO:0004813
alanine-tRNA ligase activity [PMID:7761427]"
/experiment="EXISTENCE:mutant phenotype:GO:0006419
alanyl-tRNA aminoacylation [PMID:7761427]"
/note="Cytoplasmic and mitochondrial alanyl-tRNA
synthetase; required for protein synthesis; point mutation
(cdc64-1 allele) causes cell cycle arrest at G1; lethality
of null mutation is functionally complemented by human
homolog AARS; mutations in human homolog AARS are
associated with autoimmune disease
polymyositis/dermatomyositis and with hereditary
peripheral neuropathy, Charcot-Marie-Tooth (CMT) disease"
/codon_start=1
/product="alanine--tRNA ligase"
/protein_id="NP_014980.3"
/db_xref="GeneID:854513"
/db_xref="SGD:S000005862"
/translation="MTIGDKQKWTATNVRNTFLDYFKSKEHKFVKSSPVVPFDDPTLL
FANAGMNQYKPIFLGTVDPASDFYTLKRAYNSQKCIRAGGKHNDLEDVGKDSYHHTFF
EMLGNWSFGDYFKKEAITYSWTLLTEVYGIPKDRLYVTYFEGDEKLGLEPDTEARELW
KNVGVPDDHILPGNAKDNFWEMGDQGPCGPCSEIHYDRIGGRNAASLVNMDDPDVLEV
WNLVFIQFNREQDGSLKPLPAKHIDTGMGFERLVSVLQDVRSNYDTDVFTPLFERIQE
ITSVRPYSGNFGENDKDGIDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRR
GARYARKYMNYPIGNFFSTLAPTLISQVQDIFPELAKDPAFLFEILDEEEASFAKTLD
RGERLFEKYASAASKTESKTLDGKQVWRLYDTYGFPVDLTELMAEEQGLKIDGPGFEK
AKQESYEASKRGGKKDQSDLIKLNVHELSELNDAKVPKTNDEFKYGSANVEGTILKLH
DGTNFVDEITEPGKKYGIILDKTCFYAEQGGQEYDTGKIVIDDAAEFNVENVQLYNGF
VFHTGSLEEGKLSVGDKIIASFDELRRFPIKNNHTGTHILNFALKETLGNDVDQKGSL
VAPEKLRFDFSHKKAVSNEELKKVEDICNEQIKENLQVFYKEIPLDLAKSIDGVRAVF
GETYPDPVRVVSVGKPIEELLANPANEEWTKYSIEFCGGTHVNKTGDIKYFVILEESG
IAKGIRRIVAVTGTEAFEAQRLAEQFAADLDAADKLPFSPIKEKKLKELGVKLGQLSI
SVITKNELKQKFNKIEKAVKDEVKSRAKKENKQTLDEVKTFFETNENAPYLVKFIDIS
PNAKAITEAINYMKSNDSVKDKSIYLLAGNDPEGRVAHGCYISNAALAKGIDGSALAK
KVSSIIGGKAGGKGNVFQGMGDKPAAIKDAVDDLESLFKEKLSI"
ORIGIN
1 atgacgatcg gtgataagca aaaatggacc gctactaatg tccgtaatac ctttctagac
61 tatttcaaat ctaaagaaca caagtttgtc aaatcctctc cagtagttcc atttgatgat
121 ccaactttac ttttcgctaa tgccggtatg aaccaataca agcctatctt tttaggtact
181 gtcgatccag cttccgattt ctacaccttg aaaagggctt acaactctca aaagtgtatc
241 agagctggtg gtaaacacaa cgatttagaa gatgtcggta aggattctta tcatcatacc
301 ttttttgaaa tgctgggtaa ctggtcgttt ggcgactatt tcaagaagga agctattact
361 tactcatgga ctttgttgac tgaagtatac ggcattccaa aggatagatt atacgttacc
421 tattttgaag gtgatgaaaa gttaggcttg gaacctgata ctgaagcccg tgaactatgg
481 aaaaatgttg gtgttcccga tgaccatata ttacctggta atgcaaagga caacttttgg
541 gaaatgggtg accaaggccc atgtggtcct tgttctgaaa ttcactacga tagaataggt
601 ggtagaaatg ctgcttcctt ggttaatatg gacgaccctg atgtcttgga agtttggaat
661 ttggttttca ttcaattcaa cagagaacaa gacgggtctt tgaagccttt acctgccaag
721 catatcgaca caggtatggg gttcgaaaga ttggtttctg ttctgcaaga tgttagatct
781 aactatgata ccgatgtttt cacacctttg ttcgagcgta tccaagaaat cacttcagtt
841 agaccatatt ctggcaattt tggtgagaat gacaaggacg gtatcgatac tgcctacaga
901 gttttagctg atcatgtccg tacattgact tttgccttag ctgacggtgg cgttccaaac
961 aatgaaggta gaggatatgt tttgagacgc attctaagaa gaggtgcccg ttacgcccgt
1021 aaatacatga attacccaat cggtaacttt ttctccactt tggctccaac tttaatttct
1081 caggttcaag atatctttcc cgaattagcc aaggatcctg cattcctctt tgaaatcttg
1141 gatgaagaag aagcttcttt cgctaagacc ttggatcgtg gtgaaagact atttgaaaaa
1201 tatgcttctg ctgcttctaa gactgaatct aagactttag acggtaaaca agtttggaga
1261 ctttacgaca cttatggttt cccagtcgac ttgactgaat tgatggctga agaacaaggc
1321 ttgaagattg atggacccgg tttcgaaaag gccaaacaag agtcatacga agcatccaaa
1381 agaggtggta agaaggacca aagtgatttg attaagttaa acgtccatga attatccgag
1441 ttgaacgatg ctaaggtgcc caagactaat gatgaattca aatacggcag cgccaacgtc
1501 gaaggtacca ttttgaaact gcatgacggt accaactttg ttgatgagat cactgaacca
1561 ggtaagaagt acggtattat tttggataaa acatgtttct acgccgaaca aggtggtcaa
1621 gaatatgaca ctggtaaaat tgttattgat gacgctgctg agtttaatgt tgaaaatgtt
1681 caattgtata acggttttgt tttccacacc gggtctttag aagaaggtaa gttgtctgtc
1741 ggtgacaaga ttatcgcttc attcgatgaa ctacgtcgtt tccctattaa gaacaatcat
1801 actggtacac atatcttaaa ctttgctctg aaggaaactt tgggtaatga tgtcgatcaa
1861 aagggttctt tggttgcccc agaaaaattg agattcgatt tctctcataa aaaggctgtg
1921 tcaaatgaag aattgaaaaa agttgaagat atctgtaatg agcaaattaa agaaaactta
1981 caagtgtttt acaaggaaat tccattggac ttggccaaat ccattgatgg tgttcgtgct
2041 gtctttggtg agacttaccc agatccagtt cgtgtagttt ctgttggtaa gccaatcgaa
2101 gaattgttgg ccaacccagc taacgaagag tggaccaagt attctattga attttgcggt
2161 ggtacccatg tcaacaagac aggcgatatt aaatacttcg tcattttaga agagagcggt
2221 attgcaaagg gtatcagaag aattgttgct gttactggaa ctgaagcctt tgaagctcaa
2281 agattggctg aacagtttgc tgctgatttg gatgctgcag acaagctgcc gttctctcca
2341 atcaaagaaa agaagttgaa ggaacttggt gtcaaacttg gtcaactttc aatttctgtt
2401 atcacaaaaa atgaactgaa gcagaaattt aacaaaattg aaaaagcagt gaaggatgaa
2461 gttaagtcaa gggccaaaaa agaaaacaaa caaactcttg atgaagtgaa gacatttttc
2521 gaaaccaatg agaatgctcc atacctagtt aaattcattg atatttctcc aaatgctaaa
2581 gctatcactg aagcgatcaa ctacatgaaa tccaatgatt ctgtcaaaga caagtcaatc
2641 tatttattgg caggtaatga ccctgaaggt cgtgttgctc acggttgtta catctccaat
2701 gctgctttag ccaagggtat tgatggttct gcgcttgcca aaaaggtgtc cagtatcatc
2761 ggcggtaagg ctggtggtaa aggtaatgtt ttccaaggta tgggtgataa accagccgct
2821 ataaaggatg cagttgatga tttggaaagt ttgttcaagg agaagctttc catttaa
//