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GenBank: CK845133.1
FASTA Graphics
LOCUS CK845133 618 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-AE0-xm-a-08-0-UI.s10 UI-R-AE0 Rattus norvegicus cDNA clone UI-R-AE0-xm-a-08-0-UI 3', mRNA sequence. ACCESSION CK845133 VERSION CK845133.1 DBLINK BioSample: SAMN00156264 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 618) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Distribution information can be found at http://genome.uiowa.edu/distribution/rat.html Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..618 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-AE0-xm-a-08-0-UI" /clone_lib="SAMN00156264 UI-R-AE0" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-AE0 library is a non-normalized library constructed from 15 dpc rat ventricle. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa. TAG_LIB=UI-R-AE0; TAG_SEQ=GTGTC; TAG_TISSUE=ventricle at 15 dpc" ORIGIN 1 tttttttttt ttttttttaa gatttctgaa tcaatttatt cttattatcc tgacagttgg 61 cagcaccaat gttaacaaac caattaaaat tagccaaatt atctatcact ttcaaagatt 121 tccaaatcaa tttattctta ttatcttgac agttggcagc accaatgtta acaaaccaat 181 taaaaattag ccaaaatcta agatagacac atgtacaaaa gatatacaaa ttaaaaccat 241 tttaaaagta atggataaca taatttgtct gtggctatac catctacatt cagaaatgat 301 tgtgaaatta aaaccaaaga taaaaactgt acagcatgaa ctctgagcga tttccatttc 361 agtctttcac tctcacacat ttagtgtctc tggtgggttg agaagagaaa cactgtgata 421 ttttgaatgc ttgtactttt cccttattga ctgttgggca tgctgtggtg ctttgaggta 481 ggtctggtga aggtccatga gacaaggctt aagactttct agggtatatc cagtcttttg 541 taccaatgac tcaggccagc tttgtcctgt gactgtgtan agagctaagt ggaaggcagc 601 tcccctcgtg ccgaattc //
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