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E0736H09-5 NIA Mouse four-cell-Embryo cDNA library (Long) Mus musculus cDNA clone NIA:E0736H09 IMAGE:30899516 5', mRNA sequence

GenBank: CN717040.1

FASTA Graphics 

LOCUS       CN717040                 583 bp    mRNA    linear   EST 08-FEB-2011
DEFINITION  E0736H09-5 NIA Mouse four-cell-Embryo cDNA library (Long) Mus
            musculus cDNA clone NIA:E0736H09 IMAGE:30899516 5', mRNA sequence.
ACCESSION   CN717040
VERSION     CN717040.1
DBLINK      BioSample: SAMN00174963
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol. 1 (3), E74 (2003)
   PUBMED   14691545
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: E0736  row: H  column: 09
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:E0736H09-5"
                     /db_xref="taxon:10090"
                     /clone="NIA:E0736H09 IMAGE:30899516"
                     /tissue_type="4-cell stage embryo"
                     /clone_lib="SAMN00174963 NIA Mouse four-cell-Embryo cDNA
                     library (Long)"
                     /dev_stage="4-cell"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6 (Invitrogen); Site_1: SalI;
                     Site_2: NotI; Mouse cDNA project by the Laboratory of
                     Genetics, National Institute on Aging (NIA), Intramural
                     Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA).
                     This is a long-transcript enriched cDNA library (Ref.
                     Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). The
                     mRNAs were extracted from a pool of 360 embryos at 4-cell
                     stage. Double-stranded cDNAs were synthesized with an
                     Oligo(dT) primer [Invitrogen:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
                     10.8ng of mRNA, treated with T4 DNA polymerase, and
                     purified by ethanol-precipitation. The cDNAs were ligated
                     to Lone-linker LL-Sal4, purified by phenol/chloroform, and
                     separated from free linkers by Centricon 100. Then, the
                     cDNAs were amplified by long-range high fidelity PCR using
                     Ex Taq polymerase (Takara) with a primer Sal4-S. The
                     products were purified by phenol/chloroform and Centricon
                     100. The cDNAs were digested with SalI and NotI enzymes
                     and cloned into SalI/NotI site of pCMV-SPORT6 plasmid
                     vector. The DH10B E. coli host was transformed with the
                     ligation mixture by the standard chemical method. The
                     average insert size is about 2.2Kb. The library was
                     constructed by Yulan Piao."
ORIGIN      
        1 ccgaagaaaa ggcaatggag caggctgaag gcaaggcaga ggagtgtgaa cactcaccgg
       61 ccaaagagag gtcggatggg aaagaggagg aagaagaagc aggcggggcc aaggatggca
      121 agcaggaatg tgaggttgag gccagctccg tgaagggtga actcaaaggg gtggagggca
      181 gtgcagaccc tgggtccaag tctgtctcag agaagggctc agaagaggac gaagaagaga
      241 agctggagga cgatgataag tcggaggagc cgtcgcagcc agaaggagcc gtctcgagag
      301 ggaagacttt cgatgaagaa agcaatgctc ctctgagcac tgctagggat gaaacccgag
      361 atggattcta catggaggat ggagatgctt cggtggctca gctccttcat gaacgaacat
      421 ttgccttctc cttttggccc aaggacagag taatgataaa ccgattagac aacatctgtg
      481 aagctgtgtt gaaaggcaaa tggccagtaa atcggcgcca gatgtttgat ttccagggcc
      541 ttgtccctgg gtacccacct tccgccgtgg acagtcccct gca
//
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