LOCUS CN717040 583 bp mRNA linear EST 08-FEB-2011
DEFINITION E0736H09-5 NIA Mouse four-cell-Embryo cDNA library (Long) Mus
musculus cDNA clone NIA:E0736H09 IMAGE:30899516 5', mRNA sequence.
ACCESSION CN717040
VERSION CN717040.1
DBLINK BioSample: SAMN00174963
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 583)
AUTHORS Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
TITLE Transcriptome analysis of mouse stem cells and early embryos
JOURNAL PLoS Biol. 1 (3), E74 (2003)
PUBMED 14691545
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: E0736 row: H column: 09
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..583
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:E0736H09-5"
/db_xref="taxon:10090"
/clone="NIA:E0736H09 IMAGE:30899516"
/tissue_type="4-cell stage embryo"
/clone_lib="SAMN00174963 NIA Mouse four-cell-Embryo cDNA
library (Long)"
/dev_stage="4-cell"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6 (Invitrogen); Site_1: SalI;
Site_2: NotI; Mouse cDNA project by the Laboratory of
Genetics, National Institute on Aging (NIA), Intramural
Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA).
This is a long-transcript enriched cDNA library (Ref.
Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). The
mRNAs were extracted from a pool of 360 embryos at 4-cell
stage. Double-stranded cDNAs were synthesized with an
Oligo(dT) primer [Invitrogen:
5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
10.8ng of mRNA, treated with T4 DNA polymerase, and
purified by ethanol-precipitation. The cDNAs were ligated
to Lone-linker LL-Sal4, purified by phenol/chloroform, and
separated from free linkers by Centricon 100. Then, the
cDNAs were amplified by long-range high fidelity PCR using
Ex Taq polymerase (Takara) with a primer Sal4-S. The
products were purified by phenol/chloroform and Centricon
100. The cDNAs were digested with SalI and NotI enzymes
and cloned into SalI/NotI site of pCMV-SPORT6 plasmid
vector. The DH10B E. coli host was transformed with the
ligation mixture by the standard chemical method. The
average insert size is about 2.2Kb. The library was
constructed by Yulan Piao."
ORIGIN
1 ccgaagaaaa ggcaatggag caggctgaag gcaaggcaga ggagtgtgaa cactcaccgg
61 ccaaagagag gtcggatggg aaagaggagg aagaagaagc aggcggggcc aaggatggca
121 agcaggaatg tgaggttgag gccagctccg tgaagggtga actcaaaggg gtggagggca
181 gtgcagaccc tgggtccaag tctgtctcag agaagggctc agaagaggac gaagaagaga
241 agctggagga cgatgataag tcggaggagc cgtcgcagcc agaaggagcc gtctcgagag
301 ggaagacttt cgatgaagaa agcaatgctc ctctgagcac tgctagggat gaaacccgag
361 atggattcta catggaggat ggagatgctt cggtggctca gctccttcat gaacgaacat
421 ttgccttctc cttttggccc aaggacagag taatgataaa ccgattagac aacatctgtg
481 aagctgtgtt gaaaggcaaa tggccagtaa atcggcgcca gatgtttgat ttccagggcc
541 ttgtccctgg gtacccacct tccgccgtgg acagtcccct gca
//