LOCUS AA004812 416 bp mRNA linear EST 28-JAN-2011
DEFINITION zh94a06.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
clone IMAGE:428914 3', mRNA sequence.
ACCESSION AA004812
VERSION AA004812.1
DBLINK BioSample: SAMN00155062
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 416)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 687 Std Error: 0.00
Seq primer: mob.REGA+ET.
FEATURES Location/Qualifiers
source 1..416
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:1328683"
/db_xref="taxon:9606"
/clone="IMAGE:428914"
/sex="male"
/clone_lib="SAMN00155062
Soares_fetal_liver_spleen_1NFLS_S1"
/dev_stage="20 week-post conception fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
This is a subtracted version of the original Soares fetal
liver spleen 1NFLS library. 1st strand cDNA was primed
with a Pac I - oligo(dT) primer [5'
AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Pac I and cloned into the Pac I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN
1 aaatggaaac atattttttt aaatgtatga actgaaaaat actctcccaa aatctcttca
61 ccattctact gagttgaaga gggtaagctt actgattaaa tgacagtgac tctgaagagg
121 aggcatggct gcggtcagga tgaaatgaac aaacagtcaa ccttgcaact ctctttccca
181 tataatctgg aagttgatgt ctgcaacctg gtctgatttg cccagtctct ctgatacgcc
241 tctccacaca gagcccatac ctcacatttc ttcaggcatt cctggctatc tgatggcaaa
301 cactgcctta ttgataaaga cattttccta agaaaataaa tctgtgcatt taggtggaaa
361 gatttgttcc tctctgttat tcaagtgaag aaaatggtac ctggctgcca tgtggt
//