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zh94a06.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:428914 3', mRNA sequence

GenBank: AA004812.1

FASTA Graphics 

LOCUS       AA004812                 416 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  zh94a06.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
            clone IMAGE:428914 3', mRNA sequence.
ACCESSION   AA004812
VERSION     AA004812.1
DBLINK      BioSample: SAMN00155062
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 687   Std Error: 0.00
            Seq primer: mob.REGA+ET.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1328683"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:428914"
                     /sex="male"
                     /clone_lib="SAMN00155062
                     Soares_fetal_liver_spleen_1NFLS_S1"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     This is a subtracted version of the original Soares fetal
                     liver spleen 1NFLS library. 1st strand cDNA was primed
                     with a Pac I - oligo(dT) primer [5'
                     AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 aaatggaaac atattttttt aaatgtatga actgaaaaat actctcccaa aatctcttca
       61 ccattctact gagttgaaga gggtaagctt actgattaaa tgacagtgac tctgaagagg
      121 aggcatggct gcggtcagga tgaaatgaac aaacagtcaa ccttgcaact ctctttccca
      181 tataatctgg aagttgatgt ctgcaacctg gtctgatttg cccagtctct ctgatacgcc
      241 tctccacaca gagcccatac ctcacatttc ttcaggcatt cctggctatc tgatggcaaa
      301 cactgcctta ttgataaaga cattttccta agaaaataaa tctgtgcatt taggtggaaa
      361 gatttgttcc tctctgttat tcaagtgaag aaaatggtac ctggctgcca tgtggt
//
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