LOCUS AA026153 436 bp mRNA linear EST 28-JAN-2011
DEFINITION zj99c12.r1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone
IMAGE:469174 5', mRNA sequence.
ACCESSION AA026153
VERSION AA026153.1
DBLINK BioSample: SAMN00155083
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 436)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 623 Std Error: 0.00
Seq primer: -28M13 rev2 from Amersham
High quality sequence stop: 319.
FEATURES Location/Qualifiers
source 1..436
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3755176"
/db_xref="taxon:9606"
/clone="IMAGE:469174"
/sex="female"
/clone_lib="SAMN00155083 Soares_pregnant_uterus_NbHPU"
/dev_stage="adult"
/lab_host="DH10B"
/note="Organ: uterus; Vector: pT7T3D-PacI; Site_1: Not I;
Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
oligo(dT) primer [5'
AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by M. Fatima Bonaldo."
ORIGIN
1 cagccccctc tgcagctctt catgggggtc cactcaagtc agaagccaca tctgggtgtg
61 attcagagag ctgtttatgc aaaaccagcc ttgtcaccat caggcaaggc acctgaattc
121 ccaccgcaga gagtgctgtg gncctgagga cttttctgtc accagccatt tccaggtaga
181 cccagtgtaa tcacaagcat ccttgtaagt ggcaaagagg cagaggagac agcgccagag
241 aggcagctgc atgagaagga tcaagatgtt gctgactttg aaagttggaa gaatgaaggc
301 catgagcctc agtaatgtgg gcggcttcta gaagctngaa ggggcaagga agatgggatt
361 tgacccttca gcctcccagg gggcagcttg taccttgcca tcactgattt gtagcctggg
421 tgaaggcctg ttttag
//