LOCUS AA029089 452 bp mRNA linear EST 28-JAN-2011
DEFINITION zk11c12.r1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone
IMAGE:470230 5', mRNA sequence.
ACCESSION AA029089
VERSION AA029089.1
DBLINK BioSample: SAMN00155083
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 452)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1349 Std Error: 0.00
Seq primer: -28M13 rev2 from Amersham
High quality sequence stop: 333.
FEATURES Location/Qualifiers
source 1..452
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3756232"
/db_xref="taxon:9606"
/clone="IMAGE:470230"
/sex="female"
/clone_lib="SAMN00155083 Soares_pregnant_uterus_NbHPU"
/dev_stage="adult"
/lab_host="DH10B"
/note="Organ: uterus; Vector: pT7T3D-PacI; Site_1: Not I;
Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
oligo(dT) primer [5'
AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by M. Fatima Bonaldo."
ORIGIN
1 tcagatattg ccatactgaa cctcattcta aactggtgct gtggatagtc tttccctctc
61 ccctcctgtt ttagtttaag gaaaggtttc cttcatggaa aagcactttt aattttgaga
121 atttcacatt taaaataagc caaaagcctg atgttcacat tgtgctttta aactccgtaa
181 cagctgaata caacataaca tagtctccct tgaaattccc tccctttttc agtagaggat
241 aaatagggtg actccagctg ttggaatgag aatggggata cccccaaaat aagattcttg
301 gtgaagaaaa agtcttgtac taagttcccc ttttaggtgt agccaagtct atnatatnat
361 tatgatcatg gaatagactc cnagaatcca caaagagaag aggggcttgg atgtcaaaaa
421 ttcttccatt gtggggtagg tggtcngggg at
//