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mp53g02.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:572978 5', mRNA sequence

GenBank: AA117093.1

FASTA Graphics 

LOCUS       AA117093                 436 bp    mRNA    linear   EST 17-FEB-1997
DEFINITION  mp53g02.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:572978
            5', mRNA sequence.
ACCESSION   AA117093
VERSION     AA117093.1
DBLINK      BioSample: SAMN00155149
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:347626
            Seq primer: -28M13 rev2 from Amersham
            High quality sequence stop: 429.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:572978"
                     /sex="male"
                     /tissue_type="Thymus"
                     /clone_lib="SAMN00155149 Soares_thymus_2NbMT"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through two
                     rounds of normalization, and was constructed by Bento
                     Soares and M.Fatima Bonaldo."
ORIGIN      
        1 cttgaaagga cctttacgga aaaaacgggc ctatgtggaa atagagtatg aacaggagac
       61 agagcccatg gccaaagtca aagccacatg agttcccttc acactggcag ctgtgctctt
      121 gtgtgttctg tttcctaaag tatttatatt gttaatattt atgtcacttc agtggagccg
      181 aaatctggag tgagtatgaa gagccttgag ttgagaggcg tatttttata acacatgctg
      241 taacagcttg agcccaagga atctaacaga tgaattcctg gagctggaat ggcactggct
      301 gtaaatattt ctaaattact tagaaatgtc aggatttcaa tttttttctc actcaacttt
      361 tcttatgtgg acaaatagaa gagataaaaa tttcaactta taatatttta ataccaaaat
      421 agatttttac ccccat
//
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