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nc06a02.r1 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007306, mRNA sequence

GenBank: AA244287.1

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LOCUS       AA244287                 134 bp    mRNA    linear   EST 04-JAN-2011
DEFINITION  nc06a02.r1 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007306, mRNA
            sequence.
ACCESSION   AA244287
VERSION     AA244287.1
DBLINK      BioSample: SAMN00155176
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 134)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: W. Marston Linehan, M.D., Rodrigo Chuaqui,
            M.D., Michael Emmert-Buck, M.D., Ph.D.
            cDNA Library Preparation: David B. Krizman, Ph.D.
            cDNA Library Arrayed by: Genome Systems Inc., Greg Lennon, Ph.D.
            DNA Sequencing by: Washington University Genome Sequencing Center
            Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 799   Std Error: 0.00
            Seq primer: -28m13 rev1 ET from Amersham
            High quality sequence stop: 90.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1007306"
                     /sex="male"
                     /clone_lib="SAMN00155176 NCI_CGAP_Pr1"
                     /dev_stage="45 years old"
                     /lab_host="DH10B"
                     /note="Vector: pAMP10; Site_1: Not1; Site_2: EcoRI; 1st
                     strand cDNA was primed with oligo(dT)17 on 50 ng of
                     DNAse-treated, total cellular RNA obtained from
                     5,000-10,000 microdissected, histologically normal
                     prostate epithelial cells. Double-stranded cDNA was
                     ligated to EcoRI adaptors, 5 cycles of PCR applied to the
                     cDNA with an adaptor-specific primer, and the resulting
                     PCR product subcloned into pAMP10 by the UDG-cloning
                     method (Life Technologies). Average insert size is 600 bp.
                     NOTE: Not directionally cloned. This library was
                     constructed by David Krizman."
ORIGIN      
        1 tcgaccatcc agatacacct aaggtattag taaagcggtg cattaagctg aaatatctgg
       61 cttgtgcaaa cttggggcat ttgtacctta tgtagaattc caaaatctgc ttaggatttt
      121 ccctgtccac tgga
//
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