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GenBank: AA255811.1
FASTA Graphics
LOCUS AA255811 408 bp mRNA linear EST 06-AUG-1997 DEFINITION zr84g02.r1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:682418 5', mRNA sequence. ACCESSION AA255811 VERSION AA255811.1 DBLINK BioSample: SAMN00155192 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 408) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-Merck EST Project 1997 JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 781 Std Error: 0.00 Seq primer: -28m13 rev2 ET from Amersham. FEATURES Location/Qualifiers source 1..408 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:682418" /tissue_type="Pooled human melanocyte, fetal heart, and pregnant uterus" /clone_lib="SAMN00155192 Soares_NhHMPu_S1" /lab_host="DH10B" /note="Organ: mixed (see below); Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from three normalized libraries (melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479." ORIGIN 1 gtcaggcatc actgcggcta gtccggcagc gacctagatg gggtccaccc cattcctgct 61 caagcatggg cacctaccac atggtttctg ctgctcagcc tgcctgcaac tcacctcgaa 121 ggcggaccag cctgcctctg tgatgactgc agcagacctc cttgggtgta ccaatgcccc 181 tcatctccca ctttcacacc taaccctgac tccttcacca agaagacggg agcggcagcc 241 aggagttccc gtggcacctc tctctcttcg tggctccctg cttccccctt ccctctttcc 301 gaggaagggt caacctattc tctctcaaag ccaaccccta ggccaattgc ctggatctcc 361 tcccctctcc cttctttaaa cgagcttgcc tccctcctgc caagtttg //
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