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GenBank: AA262354.1
FASTA Graphics
LOCUS AA262354 346 bp mRNA linear EST 18-MAR-1997 DEFINITION zr44g06.s1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:666298 3', mRNA sequence. ACCESSION AA262354 VERSION AA262354.1 DBLINK BioSample: SAMN00155192 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 346) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -41m13 fwd. ET from Amersham High quality sequence stop: 295. FEATURES Location/Qualifiers source 1..346 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:666298" /tissue_type="Pooled human melanocyte, fetal heart, and pregnant uterus" /clone_lib="SAMN00155192 Soares_NhHMPu_S1" /lab_host="DH10B" /note="Organ: mixed (see below); Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from three normalized libraries (melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479." ORIGIN 1 taacagtagg aaaaaccaca ctattaaagt ataaaatttt gtcaaggctc tattttctaa 61 gcctatataa aggccaggta gtaaatattt tgagctttgc ggcccatgtg atctctacta 121 cgagtactca accctgctcc agtaatatga aagtagtcac agacaactgg aaatgaatgg 181 atatggctgt atttcaataa aatattactt acaaaaatag caggaccaac acttgctgac 241 ccctcacttt cataggtttt ataaccttat taacttttaa aaggtagttc tacaacctct 301 caaatgagaa tgaaaatgaa gacaaagcta ctttagtgtt ttaaag //
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