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zr44g06.s1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:666298 3', mRNA sequence

GenBank: AA262354.1

FASTA Graphics 

LOCUS       AA262354                 346 bp    mRNA    linear   EST 18-MAR-1997
DEFINITION  zr44g06.s1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:666298
            3', mRNA sequence.
ACCESSION   AA262354
VERSION     AA262354.1
DBLINK      BioSample: SAMN00155192
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 295.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:666298"
                     /tissue_type="Pooled human melanocyte, fetal heart, and
                     pregnant uterus"
                     /clone_lib="SAMN00155192 Soares_NhHMPu_S1"
                     /lab_host="DH10B"
                     /note="Organ: mixed (see below); Vector: pT7T3D-PacI;
                     Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid
                     DNA from three normalized libraries (melanocyte 2NbHM,
                     pregnant uterus NbHPU, and fetal heart NbHH19W) were
                     mixed, and ss circles were made in vitro. Following HAP
                     purification, this DNA was used as tracer in a subtractive
                     hybridization reaction. The driver was PCR-amplified cDNAs
                     from pools of 5,000 clones made from the same 3 libraries.
                     The pools consisted of I.M.A.G.E. clones 260232-265223,
                     340488-345479, and 484488-489479."
ORIGIN      
        1 taacagtagg aaaaaccaca ctattaaagt ataaaatttt gtcaaggctc tattttctaa
       61 gcctatataa aggccaggta gtaaatattt tgagctttgc ggcccatgtg atctctacta
      121 cgagtactca accctgctcc agtaatatga aagtagtcac agacaactgg aaatgaatgg
      181 atatggctgt atttcaataa aatattactt acaaaaatag caggaccaac acttgctgac
      241 ccctcacttt cataggtttt ataaccttat taacttttaa aaggtagttc tacaacctct
      301 caaatgagaa tgaaaatgaa gacaaagcta ctttagtgtt ttaaag
//
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