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zs84e03.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:704188 3', mRNA sequence

GenBank: AA279315.1

FASTA Graphics 

LOCUS       AA279315                 185 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  zs84e03.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:704188 3',
            mRNA sequence.
ACCESSION   AA279315
VERSION     AA279315.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 185)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 2674   Std Error: 0.00
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 177.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:704188"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttcatcctc ttttgttaaa aacagactag caccactgtg acgcgtgcca cgagacacca
       61 agacggccca gtcacagagg aaaaacccga acacgacaca gaactgagga gagtccaggc
      121 ctgggctggt cgggggccac acccgcgtgg ccctggttgc tgggaggcat ggggctcagg
      181 cctgg
//
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