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GenBank: AA279315.1
FASTA Graphics
LOCUS AA279315 185 bp mRNA linear EST 08-JAN-2011 DEFINITION zs84e03.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:704188 3', mRNA sequence. ACCESSION AA279315 VERSION AA279315.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 185) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 2674 Std Error: 0.00 Seq primer: -41m13 fwd. ET from Amersham High quality sequence stop: 177. FEATURES Location/Qualifiers source 1..185 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:704188" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttcatcctc ttttgttaaa aacagactag caccactgtg acgcgtgcca cgagacacca 61 agacggccca gtcacagagg aaaaacccga acacgacaca gaactgagga gagtccaggc 121 ctgggctggt cgggggccac acccgcgtgg ccctggttgc tgggaggcat ggggctcagg 181 cctgg //
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