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GenBank: AA279938.1
FASTA Graphics
LOCUS AA279938 377 bp mRNA linear EST 08-JAN-2011 DEFINITION zs92g08.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:704990 5', mRNA sequence. ACCESSION AA279938 VERSION AA279938.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 377) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 810 Std Error: 0.00 Seq primer: -28m13 rev2 ET from Amersham High quality sequence stop: 361. FEATURES Location/Qualifiers source 1..377 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:704990" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 agattcggca ctgaggagga aatcctgagt ttttagctac ggttcggtgt gcactaagtc 61 atcttcagaa gttggtagga ttctgtgagt ccagaggaca gagcttatag aggcattagt 121 attgtaggac taattgtgga tcctgggtgc cctgttaaag taacccttgt catcatttat 181 gctaaagatg atacccttct ggcagtttgc cacagtacaa ttcagatgca tctgctttta 241 acccttttcc gttatcattg gaagtctgac agtgcgctgc ataaacacct tgagtaccac 301 agcattccgt aatatcaatt tctagaagaa gcctctttct aatagtagtg tggccggtgc 361 ttattaatca tctagct //
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