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zs92g08.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:704990 5', mRNA sequence

GenBank: AA279938.1

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LOCUS       AA279938                 377 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  zs92g08.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:704990 5',
            mRNA sequence.
ACCESSION   AA279938
VERSION     AA279938.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 377)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 810   Std Error: 0.00
            Seq primer: -28m13 rev2 ET from Amersham
            High quality sequence stop: 361.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:704990"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 agattcggca ctgaggagga aatcctgagt ttttagctac ggttcggtgt gcactaagtc
       61 atcttcagaa gttggtagga ttctgtgagt ccagaggaca gagcttatag aggcattagt
      121 attgtaggac taattgtgga tcctgggtgc cctgttaaag taacccttgt catcatttat
      181 gctaaagatg atacccttct ggcagtttgc cacagtacaa ttcagatgca tctgctttta
      241 acccttttcc gttatcattg gaagtctgac agtgcgctgc ataaacacct tgagtaccac
      301 agcattccgt aatatcaatt tctagaagaa gcctctttct aatagtagtg tggccggtgc
      361 ttattaatca tctagct
//
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