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GenBank: AA428061.1
FASTA Graphics
LOCUS AA428061 440 bp mRNA linear EST 16-OCT-1997 DEFINITION zw54h12.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:773927 3', mRNA sequence. ACCESSION AA428061 VERSION AA428061.1 DBLINK BioSample: SAMN00155442 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 440) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-Merck EST Project 1997 JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -41m13 fwd. ET from Amersham High quality sequence stop: 388. FEATURES Location/Qualifiers source 1..440 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:773927" /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w" /dev_stage="8-9 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttttttttt gtcactgcaa attgtttatt aaaacacaaa gcaatggaca gtgaaaaaca 61 tcctgatttt gtactttttg gtgggagtgg ggtgggacat ggaaggggta gggtcagatg 121 gagacagccc agaaggaaag ccagctctat ccacccctac agcttccccg gccaaccagg 181 ttcaaagccc atcagttata tctcactgtg ccgctgtcaa tgcatgttgt ggctgctggc 241 aaagagttgg tttggcctcc acagagagag gatgggaaac tagggtagaa gctacaaggg 301 ctaagaactt gatgcttttt ctccagtact tggtacagaa tagagcagcc aggcccaatg 361 tggaagggtg ggcttggttt cctgacaagc caggttagac caataatggt tgaagaggct 421 gccctgggct cagaatgggg //
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