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zw54h12.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:773927 3', mRNA sequence

GenBank: AA428061.1

FASTA Graphics 

LOCUS       AA428061                 440 bp    mRNA    linear   EST 16-OCT-1997
DEFINITION  zw54h12.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
            IMAGE:773927 3', mRNA sequence.
ACCESSION   AA428061
VERSION     AA428061.1
DBLINK      BioSample: SAMN00155442
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
            Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
            White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-Merck EST Project 1997
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 388.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:773927"
                     /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w"
                     /dev_stage="8-9 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     pooled 8-9 week (total) fetus material with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttttttt gtcactgcaa attgtttatt aaaacacaaa gcaatggaca gtgaaaaaca
       61 tcctgatttt gtactttttg gtgggagtgg ggtgggacat ggaaggggta gggtcagatg
      121 gagacagccc agaaggaaag ccagctctat ccacccctac agcttccccg gccaaccagg
      181 ttcaaagccc atcagttata tctcactgtg ccgctgtcaa tgcatgttgt ggctgctggc
      241 aaagagttgg tttggcctcc acagagagag gatgggaaac tagggtagaa gctacaaggg
      301 ctaagaactt gatgcttttt ctccagtact tggtacagaa tagagcagcc aggcccaatg
      361 tggaagggtg ggcttggttt cctgacaagc caggttagac caataatggt tgaagaggct
      421 gccctgggct cagaatgggg
//
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