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zv57c04.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:757734 3', mRNA sequence

GenBank: AA436656.1

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LOCUS       AA436656                 132 bp    mRNA    linear   EST 30-MAY-1997
DEFINITION  zv57c04.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:757734
            3', mRNA sequence.
ACCESSION   AA436656
VERSION     AA436656.1
DBLINK      BioSample: SAMN00155229
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 132)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
            Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
            White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-Merck EST Project 1997
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 121.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:5978348"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:757734"
                     /sex="male"
                     /clone_lib="SAMN00155229 Soares_testis_NHT"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     Clontech Laboratories, Inc., and primed with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization to Cot5, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttctctt tctactttta tttttcaaat tttatttctg tgaaaatgta tttgtgtaca
       61 taaaaataca aatctgggta gcaactgggt atttcaagaa aacaccgtgg acagacttgt
      121 ctccattccc ac
//
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