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zv51e08.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:757190 3', mRNA sequence

GenBank: AA443958.1

FASTA Graphics 

LOCUS       AA443958                 423 bp    mRNA    linear   EST 03-JUN-1997
DEFINITION  zv51e08.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:757190
            3', mRNA sequence.
ACCESSION   AA443958
VERSION     AA443958.1
DBLINK      BioSample: SAMN00155229
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
            Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
            White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-Merck EST Project 1997
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 398.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:5977802"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:757190"
                     /sex="male"
                     /clone_lib="SAMN00155229 Soares_testis_NHT"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     Clontech Laboratories, Inc., and primed with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization to Cot5, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttcagtgtg ctaaaaattg ttttgttttg agtaaaggaa aaagatatat aaggttaaaa
       61 atcccaagtc accacagcca gaaaacagac gtaggaggtg cctcatagct gccatgcccc
      121 taccacatgt gtcagcagga agtcaggctt ttctttcaag gccaagacac tagaacagtg
      181 ctgagcaggg taggtcagga ggcatttact ctcatgggca ttgcgttgca ggctcactca
      241 cccagacagc aggagaaaca ggccatggaa gacaacacgt ggtctacaga ctcataagta
      301 gttctctgcc tctccagttt taagatggcc ataagcatgg ccaccctcac ccacaagaag
      361 gtcagaggta ggtagagagg agggagctga cacacaggca cccaggacac cctgcttcag
      421 ggc
//
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